Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • McKinnie, S.M.; Rodriguez-Lopez, E.M.; Vederas, J.C.; Crowther, J.M.; Suzuki, H.; Dobson, R.C.; Leustek, T.; Triassi, A.J.; Wheatley, M.S.; Hudson, A.O.
    Differential response of orthologous L,L-diaminopimelate aminotransferases (DapL) to enzyme inhibitory antibiotic lead compounds (2014), Bioorg. Med. Chem., 22, 523-530.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.83 expression in Escheirchia coli Leptospira interrogans
2.6.1.83 expression in Escheirchia coli Chlamydomonas reinhardtii
2.6.1.83 expression in Escheirchia coli Verrucomicrobium spinosum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.83 modeling of structure based on the structure of the Arabidopsis thaliana enzyme and comparison between strucutre of Arabidopsis thaliana, from Leptospira interrogans, Verrucomicrobium spinosum and Chlamydomonas reinhardtii enzymes Leptospira interrogans
2.6.1.83 modeling of structure based on the structure of the Arabidopsis thaliana enzyme and comparison between strucutre of Arabidopsis thaliana, from Leptospira interrogans, Verrucomicrobium spinosum and Chlamydomonas reinhardtii enzymes Chlamydomonas reinhardtii
2.6.1.83 modeling of structure based on the structure of the Arabidopsis thaliana enzyme and comparison between strucutre of Arabidopsis thaliana, from Leptospira interrogans, Verrucomicrobium spinosum and Chlamydomonas reinhardtii enzymes Verrucomicrobium spinosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.83 (2-[(E)-[1-(4-methylphenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)acetic acid
-
 Chlamydomonas reinhardtii
2.6.1.83 (2-[(E)-[1-(4-methylphenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)acetic acid
-
 Leptospira interrogans
2.6.1.83 (2-[(E)-[1-(4-methylphenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)acetic acid
-
 Verrucomicrobium spinosum
2.6.1.83 (5Z)-5-[5-(2-chlorobenzyl)-2-hydroxybenzylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
 Chlamydomonas reinhardtii
2.6.1.83 (5Z)-5-[5-(2-chlorobenzyl)-2-hydroxybenzylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
 Leptospira interrogans
2.6.1.83 (5Z)-5-[5-(2-chlorobenzyl)-2-hydroxybenzylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
 Verrucomicrobium spinosum
2.6.1.83 2-thioxo-5-[(1,2,5-trimethyl-1H-pyrrol-3-yl)methylidene]dihydropyrimidine-4,6(1H,5H)-dione
-
 Chlamydomonas reinhardtii
2.6.1.83 2-thioxo-5-[(1,2,5-trimethyl-1H-pyrrol-3-yl)methylidene]dihydropyrimidine-4,6(1H,5H)-dione
-
 Leptospira interrogans
2.6.1.83 2-thioxo-5-[(1,2,5-trimethyl-1H-pyrrol-3-yl)methylidene]dihydropyrimidine-4,6(1H,5H)-dione
-
 Verrucomicrobium spinosum
2.6.1.83 2-[2-[(E)-(4,6-dioxo-1-phenyl-2-thioxotetrahydropyrimidin-5(2H)-ylidene)methyl]-1H-indol-1-yl]-N-phenylacetamide
-
 Chlamydomonas reinhardtii
2.6.1.83 2-[2-[(E)-(4,6-dioxo-1-phenyl-2-thioxotetrahydropyrimidin-5(2H)-ylidene)methyl]-1H-indol-1-yl]-N-phenylacetamide
-
 Leptospira interrogans
2.6.1.83 additional information comprehensive inhibition study comparing the kinetic activity of DapL orthologs using small molecule inhibitors and recombinant enzymes from Leptospira interrogans, Verrucomicrobium spinosum and Chlamydomonas reinhardtii. Enzyme active sites are conserved, although the second shell of residues close to the active site are not. The enzymes respond differently to the inhibitors Chlamydomonas reinhardtii
2.6.1.83 additional information comprehensive inhibition study comparing the kinetic activity of DapL orthologs using small molecule inhibitors and recombinant enzymes from Leptospira interrogans, Verrucomicrobium spinosum and Chlamydomonas reinhardtii. Enzyme active sites are conserved, although the second shell of residues close to the active site are not. the enzymes respond differntly to the inhibors Leptospira interrogans
2.6.1.83 additional information comprehensive inhibition study comparing the kinetic activity of DapL orthologs using small molecule inhibitors and recombinant enzymes from Leptospira interrogans, Verrucomicrobium spinosum and Chlamydomonas reinhardtii. Enzyme active sites are conserved, although the second shell of residues close to the active site are not. The enzymes respond differently to the inhibitors. 2-[2-[(E)-(4,6-dioxo-1-phenyl-2-thioxotetrahydropyrimidin-5(2H)-ylidene)methyl]-1H-indol-1-yl]-N-phenylacetamide is not inhibitory with Verrucomicrobium spinosum DapL Verrucomicrobium spinosum
2.6.1.83 N-[3-(hydrazinylcarbonyl)naphthalen-2-yl]benzenesulfonamide
-
 Chlamydomonas reinhardtii
2.6.1.83 N-[3-(hydrazinylcarbonyl)naphthalen-2-yl]benzenesulfonamide
-
 Leptospira interrogans
2.6.1.83 N-[3-(hydrazinylcarbonyl)naphthalen-2-yl]benzenesulfonamide
-
 Verrucomicrobium spinosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.83 1.1
-
 LL-2,6-Diaminoheptanedioate pH 7.6, temperature not specified in the publication Leptospira interrogans
2.6.1.83 2.7
-
 LL-2,6-Diaminoheptanedioate pH 7.6, temperature not specified in the publication Chlamydomonas reinhardtii
2.6.1.83 4
-
 LL-2,6-Diaminoheptanedioate pH 7.6, temperature not specified in the publication Verrucomicrobium spinosum

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.83 Chlamydomonas reinhardtii A8IW39
-
-
2.6.1.83 Leptospira interrogans Q72NJ3
-
-
2.6.1.83 Verrucomicrobium spinosum WP_009961032
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.83 LL-2,6-diaminoheptanedioate + 2-oxoglutarate
-
 Leptospira interrogans (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O
-
 ?
2.6.1.83 LL-2,6-diaminoheptanedioate + 2-oxoglutarate
-
 Chlamydomonas reinhardtii (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O
-
 ?
2.6.1.83 LL-2,6-diaminoheptanedioate + 2-oxoglutarate
-
 Verrucomicrobium spinosum (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.83 DapI
-
 Chlamydomonas reinhardtii
2.6.1.83 DapL
-
 Leptospira interrogans
2.6.1.83 DapL
-
 Verrucomicrobium spinosum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.83 1.6
-
 LL-2,6-Diaminoheptanedioate pH 7.6, temperature not specified in the publication Verrucomicrobium spinosum
2.6.1.83 5.7
-
 LL-2,6-Diaminoheptanedioate pH 7.6, temperature not specified in the publication Leptospira interrogans
2.6.1.83 16.4
-
 LL-2,6-Diaminoheptanedioate pH 7.6, temperature not specified in the publication Chlamydomonas reinhardtii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.6.1.83 0.005
-
 pH 7.6, temperature not specified in the publication Chlamydomonas reinhardtii N-[3-(hydrazinylcarbonyl)naphthalen-2-yl]benzenesulfonamide
2.6.1.83 0.0068
-
 pH 7.6, temperature not specified in the publication Leptospira interrogans N-[3-(hydrazinylcarbonyl)naphthalen-2-yl]benzenesulfonamide
2.6.1.83 0.025
-
 pH 7.6, temperature not specified in the publication Chlamydomonas reinhardtii 2-thioxo-5-[(1,2,5-trimethyl-1H-pyrrol-3-yl)methylidene]dihydropyrimidine-4,6(1H,5H)-dione
2.6.1.83 0.025
-
 pH 7.6, temperature not specified in the publication Leptospira interrogans 2-[2-[(E)-(4,6-dioxo-1-phenyl-2-thioxotetrahydropyrimidin-5(2H)-ylidene)methyl]-1H-indol-1-yl]-N-phenylacetamide
2.6.1.83 0.028
-
 pH 7.6, temperature not specified in the publication Leptospira interrogans 2-thioxo-5-[(1,2,5-trimethyl-1H-pyrrol-3-yl)methylidene]dihydropyrimidine-4,6(1H,5H)-dione
2.6.1.83 0.033
-
 pH 7.6, temperature not specified in the publication Chlamydomonas reinhardtii 2-[2-[(E)-(4,6-dioxo-1-phenyl-2-thioxotetrahydropyrimidin-5(2H)-ylidene)methyl]-1H-indol-1-yl]-N-phenylacetamide
2.6.1.83 0.037
-
 pH 7.6, temperature not specified in the publication Chlamydomonas reinhardtii (2-[(E)-[1-(4-methylphenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)acetic acid
2.6.1.83 0.046
-
 pH 7.6, temperature not specified in the publication Chlamydomonas reinhardtii (5Z)-5-[5-(2-chlorobenzyl)-2-hydroxybenzylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
2.6.1.83 0.047
-
 pH 7.6, temperature not specified in the publication Verrucomicrobium spinosum N-[3-(hydrazinylcarbonyl)naphthalen-2-yl]benzenesulfonamide
2.6.1.83 0.047
-
 pH 7.6, temperature not specified in the publication Verrucomicrobium spinosum (2-[(E)-[1-(4-methylphenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)acetic acid
2.6.1.83 0.048
-
 pH 7.6, temperature not specified in the publication Leptospira interrogans (5Z)-5-[5-(2-chlorobenzyl)-2-hydroxybenzylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
2.6.1.83 0.048
-
 pH 7.6, temperature not specified in the publication Leptospira interrogans (2-[(E)-[1-(4-methylphenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)acetic acid
2.6.1.83 0.057
-
 pH 7.6, temperature not specified in the publication Verrucomicrobium spinosum 2-thioxo-5-[(1,2,5-trimethyl-1H-pyrrol-3-yl)methylidene]dihydropyrimidine-4,6(1H,5H)-dione
2.6.1.83 0.25
-
 pH 7.6, temperature not specified in the publication Verrucomicrobium spinosum (5Z)-5-[5-(2-chlorobenzyl)-2-hydroxybenzylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.83 0.4
-
 LL-2,6-Diaminoheptanedioate pH 7.6, temperature not specified in the publication Verrucomicrobium spinosum
2.6.1.83 5.2
-
 LL-2,6-Diaminoheptanedioate pH 7.6, temperature not specified in the publication Leptospira interrogans
2.6.1.83 6.1
-
 LL-2,6-Diaminoheptanedioate pH 7.6, temperature not specified in the publication Chlamydomonas reinhardtii