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Literature summary extracted from

  • Liu, J.; Zou, Y.; Guan, W.; Zhai, Y.; Xue, M.; Jin, L.; Zhao, X.; Dong, J.; Wang, W.; Shen, J.; Wang, P.G.; Chen, M.
    Biosynthesis of nucleotide sugars by a promiscuous UDP-sugar pyrophosphorylase from Arabidopsis thaliana (AtUSP) (2013), Bioorg. Med. Chem. Lett., 23, 3764-3768.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.64 synthesis use of UDP-sugar pyrophosphorylase (USP) from Arabidopsis thaliana with a galactokinase from Streptococcus pneumoniae TIGR4 (SpGalK) and an inorganic diphosphatase (PPase) to effectively synthesize UDP-sugars Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.64 2.88
-
 UTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.7.7.64 3.74
-
 dUTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.7.7.64 8.14
-
 dTTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.64 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.64 4-azido-D-galactose 1-phosphate + UTP
-
 Arabidopsis thaliana UDP-4-azido-D-galactose + diphosphate 43% conversion rate ?
2.7.7.64 6-deoxy-D-galactose 1-phosphate + UTP
-
 Arabidopsis thaliana UDP-6-deoxy-D-galactose + diphosphate 29% conversion rate ?
2.7.7.64 D-galactose 1-phosphate + dTTP
-
 Arabidopsis thaliana dTDP-D-galactose + diphosphate
-
 ?
2.7.7.64 D-galactose 1-phosphate + dUTP
-
 Arabidopsis thaliana dUDP-D-galactose + diphosphate
-
 ?
2.7.7.64 D-galactose 1-phosphate + UTP
-
 Arabidopsis thaliana UDP-D-galactose + diphosphate 95% conversion rate ?
2.7.7.64 D-glucose 1-phosphate + UTP
-
 Arabidopsis thaliana UDP-D-glucose + diphosphate 28% conversion rate ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.64 USP
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.64 27
-
 dTTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.7.7.64 219
-
 dUTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.7.7.64 348
-
 UTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.64 3.3
-
 dTTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.7.7.64 59
-
 dUTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.7.7.64 121
-
 UTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana