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Literature summary extracted from

  • Forero-Baena, N.; Sanchez-Lancheros, D.; Buitrago, J.; Bustos, V.; Ramirez-Hernandez, M.
    Identification of a nicotinamide/nicotinate mononucleotide adenylyltransferase in Giardia lamblia (GlNMNAT) (2015), Biochim. Open, 1, 61-69.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.1 genes glnmnat-a and glnmnat-b, encoded on chromosome 3 and 5, respectively, DNA and amino acid sequence determination and analysis, phylogenetic analysis of GlNMNAT isoenzymes, sequence comparisons to human isozymes, recombinant expression of His-tagged glnmnat-a gene product in Escherichia coli strain BL21 Giardia intestinalis
2.7.7.18 genes glnmnat-a and glnmnat-b, encoded on chromosome 3 and 5, respectively, DNA and amino acid sequence determination and analysis, phylogenetic analysis of GlNMNAT isoenzymes, sequence comparisons to human isozymes, recombinant expression of His-tagged glnmnat-a gene product in Escherichia coli strain BL21 Giardia intestinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.1 additional information
-
 additional information Michaelis-Menten kinetics are observed for NMN and ATP, but saturation is not accomplished with NAMN, implying low affinity yet detectable activity with this substrate. Double-reciprocal plots show no cooperativity for this enzyme Giardia intestinalis
2.7.7.1 1.48 1.52 ATP pH 7.5, 26°C, recombinant isozyme GlNMNAT-A Giardia intestinalis
2.7.7.1 2.53 2.72 nicotinamide ribonucleotide pH 7.5, 26°C, recombinant isozyme GlNMNAT-A Giardia intestinalis
2.7.7.18 additional information
-
 additional information Michaelis-Menten kinetics are observed for NMN and ATP, but saturation is not accomplished with NAMN, implying low affinity yet detectable activity with this substrate. Double-reciprocal plots show no cooperativity for this enzyme Giardia intestinalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.1 Mg2+ required Giardia intestinalis
2.7.7.18 Mg2+ required Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.1 25400
-
-
 Giardia intestinalis
2.7.7.1 28100
-
-
 Giardia intestinalis
2.7.7.18 25400
-
-
 Giardia intestinalis
2.7.7.18 28100
-
-
 Giardia intestinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide Giardia intestinalis
-
 diphosphate + NAD+
-
 ?
2.7.7.1 additional information Giardia intestinalis bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 ?
-
 ?
2.7.7.18 ATP + nicotinate beta-D-ribonucleotide Giardia intestinalis
-
 diphosphate + deamido-NAD+
-
 ?
2.7.7.18 additional information Giardia intestinalis bifunctional enzyme, that also shows nicotinamide-nucleotide adenylyltransferase activity, EC 2.7.7.1 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Giardia intestinalis A8BIC5 WB clone C6, genes glnmnat-a and glnmnat-b
-
2.7.7.18 Giardia intestinalis A8BIC5 WB clone C6, genes glnmnat-a and glnmnat-b
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.1 recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Giardia intestinalis
2.7.7.18 recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide
-
 Giardia intestinalis diphosphate + NAD+
-
 ?
2.7.7.1 additional information bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 Giardia intestinalis ?
-
 ?
2.7.7.18 ATP + nicotinate beta-D-ribonucleotide
-
 Giardia intestinalis diphosphate + deamido-NAD+
-
 ?
2.7.7.18 additional information bifunctional enzyme, that also shows nicotinamide-nucleotide adenylyltransferase activity, EC 2.7.7.1 Giardia intestinalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.1 ? x * 25400, about, isozyme GlNMNAT-A, sequence calculation, x * 28100, about, isozyme GlNMNAT-B, sequence calculation Giardia intestinalis
2.7.7.1 More GlNMNAT tertiary structure model with a Rossmann-type fold, overview Giardia intestinalis
2.7.7.18 ? x * 25400, about, isozyme GlNMNAT-A, sequence calculation, x * 28100, about, isozyme GlNMNAT-B, sequence calculation Giardia intestinalis
2.7.7.18 More GlNMNAT tertiary structure model with a Rossmann-type fold, overview Giardia intestinalis

Synonyms

EC Number Synonyms Comment Organism
2.7.7.1 GlNMNAT
-
 Giardia intestinalis
2.7.7.1 nicotinamide/nicotinate mononucleotide adenylyltransferase
-
 Giardia intestinalis
2.7.7.1 NMNAT
-
 Giardia intestinalis
2.7.7.18 GlNMNAT
-
 Giardia intestinalis
2.7.7.18 nicotinamide/nicotinate mononucleotide adenylyltransferase
-
 Giardia intestinalis
2.7.7.18 NMNAT
-
 Giardia intestinalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.1 26
-
 assay at Giardia intestinalis
2.7.7.18 26
-
 assay at Giardia intestinalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.1 2.41 2.44 ATP pH 7.5, 26°C, recombinant isozyme GlNMNAT-A Giardia intestinalis
2.7.7.1 2.97 3.04 nicotinamide ribonucleotide pH 7.5, 26°C, recombinant isozyme GlNMNAT-A Giardia intestinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.1 7.5
-
 assay at Giardia intestinalis
2.7.7.18 7.5
-
 assay at Giardia intestinalis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.18 ATP
-
 Giardia intestinalis

General Information

EC Number General Information Comment Organism
2.7.7.1 metabolism the enzyme is essentially involved in the NAD biosynthesis via two different pathways, overview Giardia intestinalis
2.7.7.18 metabolism the enzyme is essentially involved in the NAD biosynthesis via two different pathways, overview Giardia intestinalis