Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pineda, E.; Vazquez, C.; Encalada, R.; Nozaki, T.; Sato, E.; Hanadate, Y.; Nequiz, M.; Olivos-Garcia, A.; Moreno-Sanchez, R.; Saavedra, E.
    Roles of acetyl-CoA synthetase (ADP-forming) and acetate kinase (PPi-forming) in ATP and PPi supply in Entamoeba histolytica (2016), Biochim. Biophys. Acta, 1860, 1163-1172.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.12 gene CL6EHI_170010, DNA and amino acid sequence determination and analysis, quantitative expression analysis by qRT-PCR, recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Entamoeba histolytica

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.12 additional information constructio of an acetate kinase antisense knockout mutant strain Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.12 0.02
-
 acetyl phosphate pH 7.0, 37°C, recombinant enzyme Entamoeba histolytica
2.7.2.12 0.07
-
 acetyl phosphate pH 6.0, 37°C, recombinant enzyme Entamoeba histolytica
2.7.2.12 2.2
-
 phosphate pH 7.0, 37°C, recombinant enzyme Entamoeba histolytica
2.7.2.12 2.6
-
 phosphate pH 6.0, 37°C, recombinant enzyme Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.12 45000
-
-
 Entamoeba histolytica
2.7.2.12 77000
-
-
 Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.12 phosphate + acetyl phosphate Entamoeba histolytica
-
 diphosphate + acetate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.12 Entamoeba histolytica C4M1C3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.12 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.12 amoeba
-
 Entamoeba histolytica
-
2.7.2.12 trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.12 diphosphate + acetate
-
 Entamoeba histolytica phosphate + acetyl phosphate
-
 r
2.7.2.12 phosphate + acetyl phosphate
-
 Entamoeba histolytica diphosphate + acetate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.12 monomer 1 * 45000, about, sequence calculation, x * 77000, about, recombinant His6-tagged enzyme, SDS-PAGE Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
2.7.2.12 ACK
-
 Entamoeba histolytica
2.7.2.12 PPi-forming acetate kinase
-
 Entamoeba histolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.12 37
-
 assay at Entamoeba histolytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.12 21
-
 acetyl phosphate pH 6.0, 37°C, recombinant enzyme Entamoeba histolytica
2.7.2.12 30
-
 acetyl phosphate pH 7.0, 37°C, recombinant enzyme Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.12 6
-
 diphosphate and acetate formation reaction, assay at Entamoeba histolytica
2.7.2.12 7
-
 acetyl phosphate formation reaction, assay at Entamoeba histolytica

General Information

EC Number General Information Comment Organism
2.7.2.12 malfunction AcK gene silencing does not affect acetate production in the parasites but promotes a slight decrease (10-20%) in the hexose phosphates and phosphate levels. Amoebae lacking ADP-forming acetyl-CoA synthetase activity are unable to reestablish the acetyl-CoA/CoA ratio after an oxidative stress challenge Entamoeba histolytica
2.7.2.12 physiological function The enzyme does not importantly contribute to ATP and phospate supply in the amoebae. Recombinant enzyme AcK shows activity only in the acetate formation direction. Enzyme AcK does not contribute to acetate production but might be marginally involved in phosphate and hexosephosphate homeostasis Entamoeba histolytica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.2.12 9
-
 phosphate pH 6.0, 37°C, recombinant enzyme Entamoeba histolytica
2.7.2.12 12
-
 phosphate pH 7.0, 37°C, recombinant enzyme Entamoeba histolytica
2.7.2.12 300
-
 acetyl phosphate pH 6.0, 37°C, recombinant enzyme Entamoeba histolytica
2.7.2.12 1500
-
 acetyl phosphate pH 7.0, 37°C, recombinant enzyme Entamoeba histolytica