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Literature summary extracted from

  • Guo, P.C.; Bao, Z.Z.; Ma, X.X.; Xia, Q.; Li, W.F.
    Structural insights into the cofactor-assisted substrate recognition of yeast methylglyoxal/isovaleraldehyde reductase Gre2 (2014), Biochim. Biophys. Acta, 1844, 1486-1492.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.265 expression in Escherichia coli Saccharomyces cerevisiae
1.1.1.283 expression in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.265 crystal structures in an apo-form at 2.00 A and NADPH-complexed form at 2.40 A resolution. Gre2 forms a homodimer, each subunit of which contains an N-terminal Rossmann-fold domain and a variable C-terminal domain, which participates in substrate recognition. The induced fit upon binding to the cofactor NADPH makes the two domains shift toward each other, producing an interdomain cleft that better fits the substrate Saccharomyces cerevisiae
1.1.1.283 crystal structures in an apo-form at 2.00 A and NADPH-complexed form at 2.40 A resolution. Gre2 forms a homodimer, each subunit of which contains an N-terminal Rossmann-fold domain and a variable C-terminal domain, which participates in substrate recognition. The induced fit upon binding to the cofactor NADPH makes the two domains shift toward each other, producing an interdomain cleft that better fits the substrate Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.265 F132A about 200% of wild-type activity Saccharomyces cerevisiae
1.1.1.265 F85A less than 0.5% of wild-type activity Saccharomyces cerevisiae
1.1.1.265 L169L complete loss of activity Saccharomyces cerevisiae
1.1.1.265 S127A complete loss of activity Saccharomyces cerevisiae
1.1.1.265 V162A about 150% of wild-type activity Saccharomyces cerevisiae
1.1.1.265 V198A about 1.5% of wild-type activity Saccharomyces cerevisiae
1.1.1.265 Y128A about 1.5% of wild-type activity Saccharomyces cerevisiae
1.1.1.265 Y128F activity similar to wild-type Saccharomyces cerevisiae
1.1.1.265 Y165A complete loss of activity Saccharomyces cerevisiae
1.1.1.265 Y165F complete loss of activity Saccharomyces cerevisiae
1.1.1.265 Y198F about 75% of wild-type activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.265 0.12
-
 3-Methylbutanal mutant Y198F, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 0.14
-
 3-Methylbutanal wild-type, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 0.18
-
 3-Methylbutanal mutant F132A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 0.2
-
 3-Methylbutanal mutant V162A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 0.23
-
 3-Methylbutanal mutant Y128F, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 0.67
-
 3-Methylbutanal mutant Y128A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 4.1
-
 3-Methylbutanal mutant Y198A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 11.1
-
 3-Methylbutanal mutant F85A, pH 7.5, 30°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.265 Saccharomyces cerevisiae Q12068 cf. EC 1.1.1.283
-
1.1.1.283 Saccharomyces cerevisiae Q12068
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.265 3-methylbutanal + NAD(P)H + H+
-
 Saccharomyces cerevisiae 3-methylbutanol + NAD(P)+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.265 Gre2
-
 Saccharomyces cerevisiae
1.1.1.283 Gre2
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.265 1.4
-
 3-Methylbutanal mutant Y128A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 5.9
-
 3-Methylbutanal mutant F85A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 9.3
-
 3-Methylbutanal mutant Y198A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 15.5
-
 3-Methylbutanal mutant Y198F, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 23.2
-
 3-Methylbutanal wild-type, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 38.9
-
 3-Methylbutanal mutant Y128F, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 52.6
-
 3-Methylbutanal mutant V162A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 62.4
-
 3-Methylbutanal mutant F132A, pH 7.5, 30°C Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.265 0.53
-
 3-Methylbutanal mutant F85A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 2.1
-
 3-Methylbutanal mutant Y128A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 2.3
-
 3-Methylbutanal mutant Y198A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 126.3
-
 3-Methylbutanal mutant Y198F, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 161
-
 3-Methylbutanal wild-type, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 166.6
-
 3-Methylbutanal mutant Y128F, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 261.5
-
 3-Methylbutanal mutant V162A, pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.265 342.7
-
 3-Methylbutanal mutant F132A, pH 7.5, 30°C Saccharomyces cerevisiae