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Literature summary extracted from

  • Bortolotti, A.; Sanchez-Azqueta, A.; Maya, C.M.; Velazquez-Campoy, A.; Hermoso, J.A.; Medina, M.; Cortez, N.
    The C-terminal extension of bacterial flavodoxin-reductases: involvement in the hydride transfer mechanism from the coenzyme (2014), Biochim. Biophys. Acta, 1837, 33-43.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.18.1.2 A266Y mutant does not allow formation of active charge-transfer complexes, probably due to restraints of C-terminus pliability. Mutant displays higher affinity for NADP+ than wild-type Rhodobacter capsulatus
1.18.1.2 A266y/Del267-272 deletion/mutation emulates the structure present in plastidic versions of the protein. It does not modify the general geometry of FAD itself, but increases exposure of the flavin to the solvent, prevents a productive geometry of FAD:NADP(H) complex and decreases the protein thermal stability. Mutant displays higher affinity for NADP+ than wild-type Rhodobacter capsulatus
1.18.1.2 Del267-272 deletion emulates the structure present in plastidic versions of the protein, mutant displays higher affinity for NADP+ than wild-type Rhodobacter capsulatus
1.19.1.1 A266Y mutant does not allow formation of active charge-transfer complexes, probably due to restraints of C-terminus pliability. Mutant displays higher affinity for NADP+ than wild-type Rhodobacter capsulatus
1.19.1.1 A266y/Del267-272 deletion/mutation emulates the structure present in plastidic versions of the protein. It does not modify the general geometry of FAD itself, but increases exposure of the flavin to the solvent, prevents a productive geometry of FAD:NADP(H) complex and decreases the protein thermal stability. Mutant displays higher affinity for NADP+ than wild-type Rhodobacter capsulatus
1.19.1.1 Del267-272 deletion emulates the structure present in plastidic versions of the protein, mutant displays higher affinity for NADP+ than wild-type Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.009
-
 NADPH mutant Del267-272, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 0.02
-
 NADPH mutant Del267-272, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 0.032
-
 NADPH mutant A266Y, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 0.039
-
 NADPH mutant A266Y/Del267-272, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 0.043
-
 NADPH mutant A266Y, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 0.043
-
 NADPH mutant A266Y/Del267-272, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 0.085
-
 NADPH wild-type, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 0.093
-
 NADPH wild-type, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 0.009
-
 NADPH mutant Del267-272, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 0.02
-
 NADPH mutant Del267-272, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 0.032
-
 NADPH mutant A266Y, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 0.039
-
 NADPH mutant A266Y/Del267-272, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 0.043
-
 NADPH mutant A266Y, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 0.043
-
 NADPH mutant A266Y/Del267-272, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 0.085
-
 NADPH wild-type, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 0.093
-
 NADPH wild-type, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Rhodobacter capsulatus Q9L6V3 enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1
-
1.19.1.1 Rhodobacter capsulatus Q9L6V3 enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 2 ferricyanide + NADPH
-
 Rhodobacter capsulatus 2 ferrocyanide + NADP+ + H+
-
 ?
1.18.1.2 2,6-dichlorophenolindophenol + NADPH
-
 Rhodobacter capsulatus reduced 2,6-dichlorophenolindophenol + NADP+
-
 ?
1.19.1.1 2 ferricyanide + NADPH
-
 Rhodobacter capsulatus 2 ferrocyanide + NADP+ + H+
-
 ?
1.19.1.1 2,6-dichlorophenolindophenol + NADPH
-
 Rhodobacter capsulatus reduced 2,6-dichlorophenolindophenol + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 1
-
 NADPH mutant Del267-272, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 7
-
 NADPH mutant A266Y, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 8
-
 NADPH mutant Del267-272, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 12
-
 NADPH mutant A266Y/Del267-272, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 20
-
 NADPH wild-type, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 68
-
 NADPH mutant A266Y, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.18.1.2 222
-
 NADPH wild-type, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 1
-
 NADPH mutant Del267-272, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 7
-
 NADPH mutant A266Y, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 8
-
 NADPH mutant Del267-272, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 12
-
 NADPH mutant A266Y/Del267-272, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 20
-
 NADPH wild-type, substrate 2,6-dichlorophenolindophenol, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 68
-
 NADPH mutant A266Y, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus
1.19.1.1 222
-
 NADPH wild-type, substrate ferricyanide, pH 7.2, 25°C Rhodobacter capsulatus