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Literature summary extracted from

  • Hansson, M.; Gustafsson, M.C.; Kannangara, C.G.; Hederstedt, L.
    Isolated Bacillus subtilis HemY has coproporphyrinogen III to coproporphyrin III oxidase activity (1997), Biochim. Biophys. Acta, 1340, 97-104.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.4 the enzyme gene is fused in its 3'-end to a polynucleotide encoding six histidine residues and expressed in both Escherichia coli BL21(DE3) cells and Bacillus subtilis 168A cells Bacillus subtilis
1.3.3.15 the enzyme gene is fused in its 3'-end to a polynucleotide encoding six histidine residues and expressed in both Escherichia coli BL21(DE3) cells and Bacillus subtilis 168A cells Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.4 53000
-
 x * 53000, SDS-PAGE Bacillus subtilis
1.3.3.15 53000
-
 x * 53000, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.4 protoporphyrinogen IX + O2 Bacillus subtilis 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III protoporphyrin IX + H2O
-
 ?
1.3.3.4 protoporphyrinogen IX + O2 Bacillus subtilis 168 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III protoporphyrin IX + H2O
-
 ?
1.3.3.15 coproporphyrinogen III + O2 Bacillus subtilis
-
 coproporphyrin III + H2O
-
 ?
1.3.3.15 coproporphyrinogen III + O2 Bacillus subtilis 168
-
 coproporphyrin III + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2 Bacillus subtilis 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III protoporphyrin IX + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2 Bacillus subtilis 168 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III protoporphyrin IX + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Bacillus subtilis P32397
-
-
1.3.3.4 Bacillus subtilis 168 P32397
-
-
1.3.3.15 Bacillus subtilis P32397
-
-
1.3.3.15 Bacillus subtilis 168 P32397
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.4 Ni2+ affinity column chromatography Bacillus subtilis
1.3.3.15 Ni2+ affinity column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 protoporphyrinogen IX + O2 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III Bacillus subtilis protoporphyrin IX + H2O
-
 ?
1.3.3.4 protoporphyrinogen IX + O2 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III Bacillus subtilis 168 protoporphyrin IX + H2O
-
 ?
1.3.3.15 coproporphyrinogen III + O2
-
 Bacillus subtilis coproporphyrin III + H2O
-
 ?
1.3.3.15 coproporphyrinogen III + O2
-
 Bacillus subtilis 168 coproporphyrin III + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III Bacillus subtilis protoporphyrin IX + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III Bacillus subtilis 168 protoporphyrin IX + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.4 ? x * 53000, SDS-PAGE Bacillus subtilis
1.3.3.15 ? x * 53000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.3.3.4 hemY
-
 Bacillus subtilis
1.3.3.4 protoporphyrinogen IX oxidase
-
 Bacillus subtilis
1.3.3.15 hemY
-
 Bacillus subtilis
1.3.3.15 protoporphyrinogen IX oxidase
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.4 FAD
-
 Bacillus subtilis
1.3.3.15 FAD
-
 Bacillus subtilis