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Literature summary extracted from

  • Gruninger, R.; Cote, C.; McAllister, T.; Abbott, D.
    Contributions of a unique beta-clamp to substrate recognition illuminates the molecular basis of exolysis in ferulic acid esterases (2016), Biochem. J., 473, 839-849.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.73 structure in both the presence and absence of ferulic acid, to 1.55 and 1.6 A resolution, respectively. Fae1A adopts an alpha/beta-hydrolase fold that is structurally conserved with bacterial FAEs, and possesses a unique loop, termed the beta-clamp, that encloses the ligand. The beta-clamp may define the structural basis of exolytic activities Anaeromyces mucronatus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 D190A about 10% residual activity with methyl ferulate Anaeromyces mucronatus
3.1.1.73 F192A about 40% residual activity with methyl ferulate Anaeromyces mucronatus
3.1.1.73 H196A about 40% residual activity with methyl ferulate Anaeromyces mucronatus
3.1.1.73 additional information deletion of the beta-clamp region, residues 185-199, complete loss of activity Anaeromyces mucronatus
3.1.1.73 Y119A about 50% residual activity with methyl ferulate Anaeromyces mucronatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Anaeromyces mucronatus F2YCB6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 2.29
-
 substrate methyl 4-coumarate, pH 7.2, 25°C Anaeromyces mucronatus
3.1.1.73 7
-
 substrate methyl sinapinate, pH 7.2, 25°C Anaeromyces mucronatus
3.1.1.73 10.6
-
 substrate methyl ferulate, pH 7.2, 25°C Anaeromyces mucronatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 methyl 4-coumarate + H2O
-
 Anaeromyces mucronatus methanol + 4-coumaric acid
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Anaeromyces mucronatus methanol + ferulic acid
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Anaeromyces mucronatus methanol + sinapic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 Fae1A
-
 Anaeromyces mucronatus