EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.15.22 | expression in Escherichia coli | Streptomyces griseolus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.15.22 | R73A/R84A | variant is also capable of converting vitamin D2 to its active form, that is, 1alpha,25-dihydroxyvitamin D2, via 25-hydroxyvitamin D2, with its 1alpha-hydroxylation activity toward 25-hydroxyvitamin D2 being much lower than that toward 25-hydroxyvitamin D3. The substitutions enhance both 25- and 26-hydroxylation activity toward vitamin D2. After 25-hydroxylation of vitamin D2, further hydroxylation at C26 may occur frequently without the release of 25-hydroxyvitamin D2 from the substrate-binding pocket | Streptomyces griseolus |
1.14.15.22 | R73V/R84A | variant is also capable of converting vitamin D2 to its active form, that is, 1alpha,25-dihydroxyvitamin D2, via 25-hydroxyvitamin D2, with its 1alpha-hydroxylation activity toward 25-hydroxyvitamin D2 being much lower than that toward 25-hydroxyvitamin D3. The substitutions enhance both 25- and 26-hydroxylation activity toward vitamin D2. After 25-hydroxylation of vitamin D2, further hydroxylation at C26 may occur frequently without the release of 25-hydroxyvitamin D2 from the substrate-binding pocket | Streptomyces griseolus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.22 | Streptomyces griseolus | P18326 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.22 | 25-hydroxyvitamin D2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Streptomyces griseolus | 1alpha,25-dihydroxyvitamin D2 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | reaction of mutants R73A/R84A and R73V/R84A | ? | |
1.14.15.22 | 25-hydroxyvitamin D2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Streptomyces griseolus | 25,26-dihydroxyvitamin D2 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | reaction of mutants R73A/R84A and R73V/R84A | ? | |
1.14.15.22 | 25-hydroxyvitamin D3 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Streptomyces griseolus | 1alpha,25-dihydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.22 | vitamin D2 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Streptomyces griseolus | 25-hydroxyvitamin D2 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.22 | vitamin D3 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Streptomyces griseolus | 25-hydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.15.22 | CYP105A1 | - |
Streptomyces griseolus |