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Literature summary extracted from

  • Sawada, N.; Sakaki, T.; Yoneda, S.; Kusudo, T.; Shinkyo, R.; Ohta, M.; Inouye, K.
    Conversion of vitamin D3 to 1alpha,25-dihydroxyvitamin D3 by Streptomyces griseolus cytochrome P450SU-1 (2004), Biochem. Biophys. Res. Commun., 320, 156-164.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.22 expression in Escherichia coli Streptomyces griseolus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.22 0.00054
-
 calciol vitamin D3 25-hydroxylation, pH 7.4, 30°C Streptomyces griseolus
1.14.15.22 0.00059
-
 vitamin D2 vitamin D2 25-hydroxylation, pH 7.4, 30°C Streptomyces griseolus
1.14.15.22 0.00091
-
 calcidiol 25-hydroxyvitamin D3 1alpha-hydroxylation, pH 7.4, 30°C Streptomyces griseolus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.22 Streptomyces griseolus P18326
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.22 recombinant protein Streptomyces griseolus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.22 calcidiol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Streptomyces griseolus calcitriol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.22 calciol + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Streptomyces griseolus calcidiol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.22 vitamin D2 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Streptomyces griseolus 25-hydroxyvitamin D2 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.22 CYP105A1
-
 Streptomyces griseolus
1.14.15.22 cytochrome P450SU-1
-
 Streptomyces griseolus
1.14.15.22 vitamin D3 dihydroxylase
-
 Streptomyces griseolus