Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Thamer, W.; Cirpus, I.; Hans, M.; Pierik, A.J.; Selmer, T.; Bill, E.; Linder, D.; Buckel, W.
    A two [4Fe-4S]-cluster-containing ferredoxin as an alternative electron donor for 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans (2003), Arch. Microbiol., 179, 197-204.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.167 Molybdenum protein contains about 0.1 molybdenum (VI) per heterodimer. The enzyme has to be activated by the extremely oxygensensitive [4Fe-4S]1+/2+-cluster-containing homodimeric component A, which generates Mo(V) by an ATP/Mg2+-induced one-electron transfer Acidaminococcus fermentans

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.167 Acidaminococcus fermentans P11569 and P11570 P11569 i.e. subunit alpha, P11570 i.e. subunit beta
-
4.2.1.167 Acidaminococcus fermentans DSM 20731 P11569 and P11570 P11569 i.e. subunit alpha, P11570 i.e. subunit beta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.167 (R)-2-hydroxyglutaryl-CoA
-
 Acidaminococcus fermentans (E)-glutaconyl-CoA + H2O
-
 ?
4.2.1.167 (R)-2-hydroxyglutaryl-CoA
-
 Acidaminococcus fermentans DSM 20731 (E)-glutaconyl-CoA + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.167 Ferredoxin alternative electron donor besides flavodoxin is a two [4Fe-4S]1+/2+-cluster-containing ferredoxin, with redox potentials of –405 mV and –340mV. The flavodoxin is the dominant electron donor protein under iron-limiting conditions. The concentration of ferredoxin increases stepwise from about 0.2 micromol/g at 7–13 microM Fe to 1.1 micromol/g at 17–45 microM Fe Acidaminococcus fermentans
4.2.1.167 flavodoxin dominant electron donor protein under iron-limiting conditions Acidaminococcus fermentans
4.2.1.167 riboflavin 5'-phosphate the actual dehydration is mediated by component D, which contains 1.0 [4Fe-4S]2+ cluster, 1.0 reduced riboflavin-5'-phosphate and about 0.1 molybdenum (VI) per heterodimer Acidaminococcus fermentans
4.2.1.167 [4Fe-4S]-center the actual dehydration is mediated by component D, which contains 1.0 [4Fe-4S]2+ cluster, 1.0 reduced riboflavin-5'-phosphate and about 0.1 molybdenum (VI) per heterodimer Acidaminococcus fermentans