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Literature summary extracted from

  • Hassan, N.; Kori, L.D.; Gandini, R.; Patel, B.K.; Divne, C.; Tan, T.C.
    High-resolution crystal structure of a polyextreme GH43 glycosidase from Halothermothrix orenii with alpha-L-arabinofuranosidase activity (2015), Acta Crystallogr. Sect. F, 71, 338-345.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expression in Escherichia coli Halothermothrix orenii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.55 structure at 1.10 A resolution. As for other GH43 members, the enzyme folds as a five-bladed -propeller. The structure features a metal-binding site on the propeller axis, near the active site Halothermothrix orenii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 Ca2+ stabilization Halothermothrix orenii
3.2.1.55 Mg2+ stabilization Halothermothrix orenii
3.2.1.55 Mn2+ pH 7.0, adding 2 mM Mn2+ increases the melting temperature of the enzyme from 63°C to 68.5°C Halothermothrix orenii
3.2.1.55 additional information divalent cation is required Halothermothrix orenii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Halothermothrix orenii B8CZV1
-
-
3.2.1.55 Halothermothrix orenii DSM 9562 B8CZV1
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 0.017
-
 presence of 4 M NaCl and Mg2+, pH 6.5, 50°C Halothermothrix orenii
3.2.1.55 0.036
-
 presence of 4 M NaCl and Mn2+, pH 6.5, 50°C Halothermothrix orenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Halothermothrix orenii 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Halothermothrix orenii DSM 9562 4-nitrophenol + alpha-L-arabinofuranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 AraF43
-
 Halothermothrix orenii
3.2.1.55 Hore_20580
-
 Halothermothrix orenii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 63
-
 melting temperature Halothermothrix orenii
3.2.1.55 68.5
-
 melting temperatue, presence of 2 mM Mn2+ Halothermothrix orenii
3.2.1.55 76
-
 melting temperature, presence of 4 M NaCl and Mn2+ at pH 6.5 Halothermothrix orenii