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Literature summary extracted from

  • Nelson, S.K.; Kelleher, A.; Robinson, G.; Reiling, S.; Asojo, O.A.
    Structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa (2013), Acta Crystallogr. Sect. F, 69, 1084-1088.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.55 gene kdo8ps, recombinant expression of His6-tagged codon-optimized enzyme in Escherichia coli strain Rosetta2 (pLysS) DE3 Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.55 purified recombinant His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.003 ml of 5 mg/ml protein in 0.2 M Tris-HCl, pH 7.4, 0.1 M NaCl, 0.05% 2-mercaptoethanol, and 0.1 mM phosphoenolpyruvate with 0.0015 ml of reservoir solution containing 0.2 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5, 18% w/v PEG 8000, 20°C, 2 days, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacementusing structure PDB ID 1g7v and model building Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O Pseudomonas aeruginosa
-
 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Pseudomonas aeruginosa
-
 gene kdo8ps
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55 recombinant His6-tagged enzyme from Escherichia coli strain Rosetta2 (pLysS) DE3 by nickel affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
-
 Pseudomonas aeruginosa 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.55 More two-dimensional structure comparison Pseudomonas aeruginosa
2.5.1.55 oligomer the enzyme structure is a prototypical alpha/beta TIM barrel structure expected from this family of enzymes and contains a tetramer in the asymmetric unit Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.5.1.55 2-dehydro-3-deoxyphosphooctonate aldolase
-
 Pseudomonas aeruginosa
2.5.1.55 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase
-
 Pseudomonas aeruginosa
2.5.1.55 KDO8PS
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
2.5.1.55 additional information active site structure, overview Pseudomonas aeruginosa
2.5.1.55 physiological function the enzyme catalyzes a key step in the synthesis of the lipopolysaccharide of most Gram-negative bacteria, it catalyzes the condensation reaction between phosphoenolpyruvate and arabinose 5-phosphate to produce 3-deoxy-D-manno-octulosonate 8-phosphate. The enzyme is vital for for proper synthesis and assembly of lipoplysaccharides and the survival and virulence of Pseudomonas aeruginosa Pseudomonas aeruginosa