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Literature summary extracted from

  • Nomme, J.; Murphy, J.M.; Su, Y.; Sansone, N.D.; Armijo, A.L.; Olson, S.T.; Radu, C.; Lavie, A.
    Structural characterization of new deoxycytidine kinase inhibitors rationalizes the affinity-determining moieties of the molecules (2014), Acta Crystallogr. Sect. D, 70, 68-78.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.74 expressed in Escherichia coli BL21 C41(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.74 in complex with inhibitors and UDP, hanging drop vapor diffusion method, using 0.9-1.5 M trisodium citrate dehydrate and 25 mM HEPES pH 7.5 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 2-[(2[-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
-
 Homo sapiens
2.7.1.74 2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-methyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
-
 Homo sapiens
2.7.1.74 2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-propyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
-
 Homo sapiens
2.7.1.74 2-[([5-ethyl-2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.74 cytosol
-
 Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 ATP + deoxycytidine Homo sapiens
-
 ADP + dCMP
-
 ?
2.7.1.74 UTP + deoxycytidine Homo sapiens
-
 UDP + dCMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens P27707
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 HisTrap nickel-affinity column chromatography and S-200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + clofarabine
-
 Homo sapiens ADP + clofarabine 5'-phosphate
-
 ?
2.7.1.74 ATP + deoxyadenosine
-
 Homo sapiens ADP + dAMP
-
 ?
2.7.1.74 ATP + deoxycytidine
-
 Homo sapiens ADP + dCMP
-
 ?
2.7.1.74 ATP + deoxyguanosine
-
 Homo sapiens ADP + dGMP
-
 ?
2.7.1.74 ATP + fludarabine
-
 Homo sapiens ADP + fludarabine 5'-phosphate
-
 ?
2.7.1.74 ATP + gemcitabine
-
 Homo sapiens ADP + gemcitabine 5'-phosphate
-
 ?
2.7.1.74 ATP + lamivudine
-
 Homo sapiens ADP + lamivudine 5'-phosphate
-
 ?
2.7.1.74 UTP + deoxyadenosine
-
 Homo sapiens UDP + dAMP
-
 ?
2.7.1.74 UTP + deoxycytidine
-
 Homo sapiens UDP + dCMP
-
 ?
2.7.1.74 UTP + deoxyguanosine
-
 Homo sapiens UDP + dGMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.74 homodimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.74 dCK
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.74 0.0000167
-
 pH and temperature not specified in the publication Homo sapiens 2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-propyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
2.7.1.74 0.0000227
-
 pH and temperature not specified in the publication Homo sapiens 2-[([5-ethyl-2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
2.7.1.74 0.0000263
-
 pH and temperature not specified in the publication Homo sapiens 2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-methyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
2.7.1.74 0.0007457
-
 pH and temperature not specified in the publication Homo sapiens 2-[(2[-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine