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Literature summary extracted from

  • Arita, T.; Miyazaki, S.; Teramoto, S.; Yoshitama, K.
    Major anthocyanin biosynthesis in the brilliant crimson petals from Erythrina crista-galli L (2014), Sci. Hortic., 168, 272-280.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.297 dithiothreitol 1 mM, 125% of initial activity Erythrina crista-galli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.297 1,10-phenanthroline 1 mM, 84% residual activity Erythrina crista-galli
2.4.1.297 4-chloromercuribenzoate 1 mM, 4% residual activity Erythrina crista-galli
2.4.1.297 Co2+ 1 mM, 31% residual activity Erythrina crista-galli
2.4.1.297 Cu2+ 1 mM, 10% residual activity Erythrina crista-galli
2.4.1.297 additional information not inhibitory: EDTA at 1 mM Erythrina crista-galli
2.4.1.297 N-ethylmaleimide 1 mM, 16.4% residual activity Erythrina crista-galli
2.4.1.297 Zn2+ 1 mM, 3.5% residual activity Erythrina crista-galli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.297 82000
-
 gel fitlration Erythrina crista-galli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.297 Erythrina crista-galli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.297 from petal Erythrina crista-galli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.297 petal
-
 Erythrina crista-galli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.297 additional information enzyme catalyzes only the 2''-O-glucosylation of flavonoid 3-O-glucosideto produce flavonoid 3-O-sophoroside. Both anthocyanidin 3-O-glucosides and flavonol 3-O-glucosides are favorable acceptor substrates of the enzyme, whereas no activity is shown toward C-glucosylflavones such as isovitexin Erythrina crista-galli ?
-
 ?
2.4.1.297 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-galactoside 9.1% of the activity with cyanidin 3-O-beta-D-glucoside Erythrina crista-galli UDP + cyanidin 2''-O-alpha-D-glucoside 3-O-beta-D-galactoside
-
 ?
2.4.1.297 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Erythrina crista-galli UDP + cyanidin 3-O-sophoroside
-
 ?
2.4.1.297 UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside 64.8% of the activity with cyanidin 3-O-beta-D-glucoside Erythrina crista-galli UDP + delphinidin 3-O-sophoroside
-
 ?
2.4.1.297 UDP-alpha-D-glucose + kaempferol 3-O-beta-D-glucoside 41.6% of the activity with cyanidin 3-O-beta-D-glucoside Erythrina crista-galli UDP + kaempferol 3-O-sophoroside
-
 ?
2.4.1.297 UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside 29.3% of the activity with cyanidin 3-O-beta-D-glucoside Erythrina crista-galli UDP + pelargonidin 3-O-sophoroside
-
 ?
2.4.1.297 UDP-alpha-D-glucose + quercetin 3-O-beta-D-glucoside 33.8% of the activity with cyanidin 3-O-beta-D-glucoside Erythrina crista-galli UDP + quercetin 3-O-sophoroside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.297 monomer 1 * 82000, SDS-PAGE Erythrina crista-galli

Synonyms

EC Number Synonyms Comment Organism
2.4.1.297 3GGT
-
 Erythrina crista-galli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.297 7.5
-
-
 Erythrina crista-galli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.297 Erythrina crista-galli isoelectric focusing
-
 6.5