BRENDA - Enzyme Database

TRNAHis 5-methylcytidine levels increase in response to several growth arrest conditions in Saccharomyces cerevisiae

Preston, M.; DSilva, S.; Kon, Y.; Phizicky, E.; RNA 19, 243-256 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.202
additional information
levels of tRNAHis m5C increase following several nutrient starvation treatments of the BY4741 strain, Trm4 protein levels do not increase during starvation conditions
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.202
S-adenosyl-L-methionine + cytosine34 in tRNA
Saccharomyces cerevisiae
cf. EC 2.1.1.203
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine34 in tRNA
Saccharomyces cerevisiae BY4741
cf. EC 2.1.1.203
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine40 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine40 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNAHis
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNAHis
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine49 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine49 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine50 in tRNAHis
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.202
Saccharomyces cerevisiae
-
-
-
2.1.1.202
Saccharomyces cerevisiae BY4741
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.202
S-adenosyl-L-methionine + cytosine34 in tRNA
cf. EC 2.1.1.203
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine34 in tRNA
cf. EC 2.1.1.203
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine40 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine40 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNAHis
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNAHis
purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNAHis
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine49 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine49 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine50 in tRNAHis
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine50 in tRNAHis
purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.202
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.202
additional information
levels of tRNAHis m5C increase following several nutrient starvation treatments of the BY4741 strain, Trm4 protein levels do not increase during starvation conditions
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.202
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.202
S-adenosyl-L-methionine + cytosine34 in tRNA
Saccharomyces cerevisiae
cf. EC 2.1.1.203
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine34 in tRNA
Saccharomyces cerevisiae BY4741
cf. EC 2.1.1.203
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine40 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine40 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNAHis
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNAHis
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine49 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine49 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine50 in tRNAHis
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.202
S-adenosyl-L-methionine + cytosine34 in tRNA
cf. EC 2.1.1.203
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine34 in tRNA
cf. EC 2.1.1.203
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine40 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine40 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNAHis
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNAHis
purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNAHis
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine49 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine49 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine50 in tRNAHis
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine50 in tRNAHis
purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
2.1.1.202
Saccharomyces cerevisiae
levels of tRNAHis m5C increase following several nutrient starvation treatments of the BY4741 strain, Trm4 protein levels do not increase during starvation conditions
additional information
General Information
EC Number
General Information
Commentary
Organism
2.1.1.202
malfunction
yeast strains depleted of tRNAHis guanylyltransferase accumulate uncharged tRNAHis lacking the G-1 residue and subsequently accumulate additional 5-methylcytidine (m5C) at residues C48 and C50 of tRNAHis, due to the activity of the m5Cmethyltransferase Trm4. The increase in tRNAHis m5C levels does not require loss of Thg1, loss of G-1 of tRNAHis, or cell death but is associated with growth arrest following different stress conditions. Substantially increased tRNAHis m5C levels occur after temperature-sensitive strains are grown at nonpermissive temperature, and after wild-type strains are grown to stationary phase, starved for required amino acids, or treated with rapamycin. More modest accumulations of m5C in tRNAHis occur after starvation for glucose and after starvation for uracil. In virtually all cases examined, the additional m5C on tRNAHis occurs while cells are fully viable, and the increase is neither due to the GCN4 pathway, nor to increased Trm4 levels, phenotypes, overview. The increased amount of m5C is specific to tRNAHis. tRNAVal(AAC), which also normally has unmodified C48 and C50 residues adjacent to m5C49, has only marginally increased levels of m5C 7 h after temperature shift in the fcp1-1ts mutant
Saccharomyces cerevisiae
2.1.1.202
metabolism
the GCN4 pathway is not responsible for additional m5C levels on tRNAHis
Saccharomyces cerevisiae
2.1.1.202
additional information
tRNAHis m5C levels are unusually responsive to yeast growth conditions
Saccharomyces cerevisiae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.202
malfunction
yeast strains depleted of tRNAHis guanylyltransferase accumulate uncharged tRNAHis lacking the G-1 residue and subsequently accumulate additional 5-methylcytidine (m5C) at residues C48 and C50 of tRNAHis, due to the activity of the m5Cmethyltransferase Trm4. The increase in tRNAHis m5C levels does not require loss of Thg1, loss of G-1 of tRNAHis, or cell death but is associated with growth arrest following different stress conditions. Substantially increased tRNAHis m5C levels occur after temperature-sensitive strains are grown at nonpermissive temperature, and after wild-type strains are grown to stationary phase, starved for required amino acids, or treated with rapamycin. More modest accumulations of m5C in tRNAHis occur after starvation for glucose and after starvation for uracil. In virtually all cases examined, the additional m5C on tRNAHis occurs while cells are fully viable, and the increase is neither due to the GCN4 pathway, nor to increased Trm4 levels, phenotypes, overview. The increased amount of m5C is specific to tRNAHis. tRNAVal(AAC), which also normally has unmodified C48 and C50 residues adjacent to m5C49, has only marginally increased levels of m5C 7 h after temperature shift in the fcp1-1ts mutant
Saccharomyces cerevisiae
2.1.1.202
metabolism
the GCN4 pathway is not responsible for additional m5C levels on tRNAHis
Saccharomyces cerevisiae
2.1.1.202
additional information
tRNAHis m5C levels are unusually responsive to yeast growth conditions
Saccharomyces cerevisiae
Expression (protein specific)
EC Number
Organism
Commentary
Expression
2.1.1.202
Saccharomyces cerevisiae
levels of tRNAHis m5C increase following several nutrient starvation treatments of the BY4741 strain, Trm4 protein levels do not increase during starvation conditions
additional information