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Literature summary extracted from

  • Gabriel, I.; Milewski, S.
    Characterization of recombinant homocitrate synthase from Candida albicans (2016), Protein Expr. Purif., 125, 7-18.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.14 expressed in Escherichia coli Rosetta (DE3) pLysS cells Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.14 2-aminoethyl-L-cysteine
-
 Candida albicans
2.3.3.14 Cu2+ strong inhibitory effect on isoform Lys22p Candida albicans
2.3.3.14 DL-5-hydroxylysine
-
 Candida albicans
2.3.3.14 DL-alpha-Difluoromethyllysine strong inhibitor of isoform Lys22p, but not Lys21p Candida albicans
2.3.3.14 EDTA strong inhibitory effect on isoform Lys22p Candida albicans
2.3.3.14 L-Arg inhibitor of isoform Lys22p, but not Lys21p Candida albicans
2.3.3.14 L-Lys competitive inhibitor Candida albicans
2.3.3.14 additional information not inhibited by L-ornithine, L-norleucine, L-leucine, L-canavanine, gamma-amino-beta-hydroxybutyric acid, gamma-amino-n-butyric acid, and O-(2-aminoethyl)-L-serine; there is little or no effect of Mg2+ or Ca2+ ions Candida albicans
2.3.3.14 S-(2-aminoethyl)-L-cysteine
-
 Candida albicans
2.3.3.14 Zn2+ strong inhibitory effect on isoform Lys22p Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.14 0.113
-
 2-oxoglutarate isoform Lys21p, at pH 7.5 and 22°C Candida albicans
2.3.3.14 0.152
-
 2-oxoglutarate isoform Lys22p, at pH 7.5 and 22°C Candida albicans
2.3.3.14 0.48
-
 acetyl-CoA isoform Lys22p, at pH 7.5 and 22°C Candida albicans
2.3.3.14 0.8
-
 acetyl-CoA isoform Lys21p, at pH 7.5 and 22°C Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.14 47087
-
 4 * 47087, isoform Lys22p, calculated from amino acid sequence Candida albicans
2.3.3.14 48300
-
 4 * 48300, isoform Lys21p, SDS-PAGE Candida albicans
2.3.3.14 48314
-
 4 * 48314, isoform Lys21p, calculated from amino acid sequence Candida albicans
2.3.3.14 49400
-
 4 * 49400, isoform Lys22p, SDS-PAGE Candida albicans
2.3.3.14 200700
-
 isoform Lys22p, gel filtration Candida albicans
2.3.3.14 210300
-
 isoform Lys21p, gel filtration Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate Candida albicans
-
 (2R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.14 Candida albicans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.14 HisTrap column chromatography Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate
-
 Candida albicans (2R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?
2.3.3.14 additional information isoforms Lys21p and Lys22p are not able to catalyze the condensation of oxaloacetate with acetyl-CoA to give citrate Candida albicans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.14 homotetramer 4 * 47087, isoform Lys22p, calculated from amino acid sequence Candida albicans
2.3.3.14 homotetramer 4 * 48300, isoform Lys21p, SDS-PAGE Candida albicans
2.3.3.14 homotetramer 4 * 48314, isoform Lys21p, calculated from amino acid sequence Candida albicans
2.3.3.14 homotetramer 4 * 49400, isoform Lys22p, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
2.3.3.14 HCS
-
 Candida albicans
2.3.3.14 LYS21
-
 Candida albicans
2.3.3.14 Lys21p isoform Candida albicans
2.3.3.14 LYS22
-
 Candida albicans
2.3.3.14 Lys22p isoform Candida albicans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.14 0.2
-
 acetyl-CoA isoform Lys21p, at pH 7.5 and 22°C Candida albicans
2.3.3.14 0.2
-
 acetyl-CoA isoform Lys22p, at pH 7.5 and 22°C Candida albicans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.3.14 0.032
-
 isoform Lys22p, at pH 7.5 and 22°C Candida albicans DL-alpha-Difluoromethyllysine
2.3.3.14 0.0541
-
 isoform Lys22p, at pH 7.5 and 22°C Candida albicans L-Lys
2.3.3.14 0.286
-
 isoform Lys21p, at pH 7.5 and 22°C Candida albicans L-Lys
2.3.3.14 1.8
-
 isoform Lys22p, at pH 7.5 and 22°C Candida albicans S-(2-aminoethyl)-L-cysteine
2.3.3.14 5.7
-
 isoform Lys22p, at pH 7.5 and 22°C Candida albicans DL-5-hydroxylysine
2.3.3.14 16.5
-
 isoform Lys21p, at pH 7.5 and 22°C Candida albicans 2-aminoethyl-L-cysteine
2.3.3.14 18.7
-
 isoform Lys22p, at pH 7.5 and 22°C Candida albicans L-Arg