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Literature summary extracted from

  • Koo, Y.; Lee, H.; Jeon, H.; Choi, H.; Choung, W.; Shim, J.
    Development and characterization of cyclodextrin glucanotransferase as a maltoheptaose-producing enzyme using site-directed mutagenesis (2015), Protein Eng. Des. Sel., 28, 531-537.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 expressed in Escherichia coli Bacillus sp. (in: Bacteria)

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.19 H233Y the mutant primarily produces maltoheptaose using beta-cyclodextrin via a hydrolysis reaction. The mutant enzyme also shows hydrolyzing activity against gamma-cyclodextrin but is unable to catalyze the hydrolysis of alpha-cyclodextrin Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus sp. (in: Bacteria) Q6S3E3
-
-
2.4.1.19 Bacillus sp. (in: Bacteria) I-5 Q6S3E3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 beta-cyclodextrin affinity column chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) beta-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) I-5 beta-cyclodextrin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 cyclodextrin glucanotransferase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 60
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 6
-
-
 Bacillus sp. (in: Bacteria)