Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tantanarat, K.; ONeill, E.; Rejzek, M.; Field, R.; Limpaseni, T.
    Expression and characterization of 4-alpha-glucanotransferase genes from Manihot esculenta Crantz and Arabidopsis thaliana and their use for the production of cycloamyloses (2014), Process Biochem., 49, 84-89.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expressed in Escherichia coli BL21 Star (DE3) One Shot cells Manihot esculenta
2.4.1.25 expressed in Escherichia coli BL21 Star (DE3) One Shot cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 27.5
-
 maltotriose at pH 7.0 and 37 °C Arabidopsis thaliana
2.4.1.25 69.2
-
 maltotriose at pH 7.0 and 37 °C Manihot esculenta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 59400
-
 2 * 59400, calculated from amino acid sequence Manihot esculenta
2.4.1.25 66000
-
 2 * 66000, SDS-PAGE Arabidopsis thaliana
2.4.1.25 117000
-
 gel filtration Manihot esculenta
2.4.1.25 123000
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Arabidopsis thaliana Q9LV91
-
-
2.4.1.25 Manihot esculenta
-
 cultivar KU50
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25 Ni HiTrap column chromatography and Superdex 75 gel filtration Manihot esculenta
2.4.1.25 Ni HiTrap column chromatography and Superdex 75 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltotriose + maltotriose
-
 Manihot esculenta maltooligosaccharides
-
 ?
2.4.1.25 maltotriose + maltotriose
-
 Arabidopsis thaliana maltooligosaccharides
-
 ?
2.4.1.25 potato amylose + glycosyl acceptor
-
 Manihot esculenta cycloamylose the enzyme can produce cycloamyloses in the range 16 to more than 60 glucose residues ?
2.4.1.25 potato amylose + glycosyl acceptor
-
 Arabidopsis thaliana cycloamylose the enzyme can produce cycloamyloses in the range 16–50 glucose residues ?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 homodimer 2 * 66000, SDS-PAGE Arabidopsis thaliana
2.4.1.25 homodimer 2 * 59400, calculated from amino acid sequence Manihot esculenta

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 4alphaGTase
-
 Manihot esculenta
2.4.1.25 4alphaGTase
-
 Arabidopsis thaliana
2.4.1.25 DPE1
-
 Manihot esculenta
2.4.1.25 DPE1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 37
-
-
 Manihot esculenta
2.4.1.25 37
-
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.25 580
-
 maltotriose at pH 7.0 and 37 °C Arabidopsis thaliana
2.4.1.25 894
-
 maltotriose at pH 7.0 and 37 °C Manihot esculenta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 6 8
-
 Manihot esculenta
2.4.1.25 6 8
-
 Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.25 Manihot esculenta calculated from amino acid sequence
-
 5.1
2.4.1.25 Arabidopsis thaliana calculated from amino acid sequence
-
 5.1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.25 12.9
-
 maltotriose at pH 7.0 and 37 °C Manihot esculenta
2.4.1.25 21.6
-
 maltotriose at pH 7.0 and 37 °C Arabidopsis thaliana