Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chiku, K.; Nihira, T.; Suzuki, E.; Nishimoto, M.; Kitaoka, M.; Ohtsubo, K.; Nakai, H.
    Discovery of two beta-1,2-mannoside phosphorylases showing different chain-length specificities from Thermoanaerobacter sp. X-514 (2014), PLoS One, 9, e114882.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.340 expression in in Escherichia coli Rosetta 2 (DE3) Thermoanaerobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.340 0.2
-
 D-fructose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 0.41
-
 alpha-D-mannose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 0.52
-
 [(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
2.4.1.340 0.63
-
 phosphate cosubstrate: [(1->2)-beta-D-mannosyl]4, pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
2.4.1.340 0.71
-
 phosphate cosubstrate: [(1->2)-beta-D-mannosyl]3, pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
2.4.1.340 0.74
-
 phosphate cosubstrate: [(1->2)-beta-D-mannosyl]2, pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
2.4.1.340 0.77
-
 [(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 0.92
-
 [(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
2.4.1.340 1.2
-
 [(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 1.4
-
 alpha-D-mannose 1-phosphate pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 2.3
-
 [(1->2)-beta-D-mannosyl]4 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.340 33997
-
 2 * 33997, calculated from sequence Thermoanaerobacter sp.
2.4.1.340 34000
-
 2 * 34000, SDS-PAGE Thermoanaerobacter sp.
2.4.1.340 50000
-
 gel filtration Thermoanaerobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.340 [(1->2)-beta-D-mannosyl]n + phosphate Thermoanaerobacter sp. involved in GDP-D-mannose biosynthesis [(1->2)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.339 Thermoanaerobacter sp.
-
-
-
2.4.1.339 Thermoanaerobacter sp. X-514
-
-
-
2.4.1.340 Thermoanaerobacter sp. B0K2C2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.340
-
 Thermoanaerobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.339 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate
-
 Thermoanaerobacter sp. D-mannopyranose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.339 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate
-
 Thermoanaerobacter sp. X-514 D-mannopyranose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.340 alpha-D-mannose + alpha-D-mannose 1-phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]2 + phosphate
-
 ?
2.4.1.340 D-fructose + alpha-D-mannose 1-phosphate
-
 Thermoanaerobacter sp. ? + phosphate
-
 ?
2.4.1.340 [(1->2)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]3 + phosphate
-
 ?
2.4.1.340 [(1->2)-beta-D-mannosyl]2 + phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. alpha-D-mannose 1-phosphate
-
 r
2.4.1.340 [(1->2)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]4 + phosphate
-
 ?
2.4.1.340 [(1->2)-beta-D-mannosyl]3 + phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate
-
 r
2.4.1.340 [(1->2)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]5 + phosphate
-
 ?
2.4.1.340 [(1->2)-beta-D-mannosyl]4 + phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate
-
 r
2.4.1.340 [(1->2)-beta-D-mannosyl]n + phosphate involved in GDP-D-mannose biosynthesis Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.340 homodimer 2 * 34000, SDS-PAGE Thermoanaerobacter sp.
2.4.1.340 homodimer 2 * 33997, calculated from sequence Thermoanaerobacter sp.

Synonyms

EC Number Synonyms Comment Organism
2.4.1.340 1,2-beta-oligomannan phosphorylase
-
 Thermoanaerobacter sp.
2.4.1.340 1,2-beta-oligomannan:phosphate alpha-D-mannosyltransferase
-
 Thermoanaerobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.340 55
-
 stable up to 55°C during a 30 min incubation Thermoanaerobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.340 1.9
-
 [(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
2.4.1.340 1.9
-
 alpha-D-mannose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 2
-
 D-fructose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 20
-
 [(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
2.4.1.340 22
-
 alpha-D-mannose 1-phosphate pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 25
-
 [(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 43
-
 [(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 49
-
 [(1->2)-beta-D-mannosyl]4 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.340 5
-
 synthetic reaction Thermoanaerobacter sp.
2.4.1.340 6
-
 phosphorolytic activity Thermoanaerobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.340 4 9.5 stable in pH-range 4.0-9.5 during a 30 min incubation Thermoanaerobacter sp.

General Information

EC Number General Information Comment Organism
2.4.1.340 metabolism involved in GDP-D-mannose biosynthesis Thermoanaerobacter sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.340 3.7
-
 [(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
2.4.1.340 4.6
-
 alpha-D-mannose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 10
-
 D-fructose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 16
-
 alpha-D-mannose 1-phosphate pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 21
-
 [(1->2)-beta-D-mannosyl]4 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
2.4.1.340 22
-
 [(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
2.4.1.340 32
-
 [(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.4.1.340 36
-
 [(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.