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Literature summary extracted from

  • Bartasun, P.; Cieslinski, H.; Bujacz, A.; Wierzbicka-Wos, A.; Kur, J.
    A study on the interaction of rhodamine B with methylthioadenosine phosphorylase protein sourced from an antarctic soil metagenomic library (2013), PLoS ONE, 8, e55697.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.28 expressed in Escherichia coli BL21(DE3)pLysS cells uncultured bacterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.28 apoenzyme and in complex with rhodamine B, hanging drop vapor diffusion method, using 35% (w/v) PPG 400, 75 mM MgCl2 and 0.1 M Bis-Tris pH 6.5 uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.28 30000
-
 3 * 30000, SDS-PAGE uncultured bacterium
2.4.2.28 32000
-
 3 * 32000, calculated from amino acid sequence uncultured bacterium
2.4.2.28 97000
-
 gel filtration uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.28 S-methyl-5'-thioadenosine + phosphate uncultured bacterium
-
 adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 uncultured bacterium C6KFA4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28 Fractogel EMD DEAE column chromatograph and Resource Q column chromatography uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 S-methyl-5'-thioadenosine + phosphate
-
 uncultured bacterium adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.28 homotrimer 3 * 30000, SDS-PAGE uncultured bacterium
2.4.2.28 homotrimer 3 * 32000, calculated from amino acid sequence uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
2.4.2.28 methylthioadenosine phosphorylase
-
 uncultured bacterium
2.4.2.28 RSFP
-
 uncultured bacterium