EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.4 | gene amsA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLyS | Synechococcus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.4 | additional information | Synechococcus sp. | the enzyme AMS exhibits multiple catalytic activities. Primarily, it can hydrolyze sucrose to glucose and fructose or transfer glucose from sucrose hydrolysis to another glucose or acceptormolecule. As a side reaction, it is also able to catalyze the isomerization of sucrose to turanose or trehalulose | ? | - |
? | |
2.4.1.4 | sucrose + [(1->4)-alpha-D-glucosyl]n | Synechococcus sp. | - |
D-fructose + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.4 | Synechococcus sp. | B1XIU7 | gene amsA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.4 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLyS by cobalt affinity chromatography | Synechococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.4 | additional information | the enzyme AMS exhibits multiple catalytic activities. Primarily, it can hydrolyze sucrose to glucose and fructose or transfer glucose from sucrose hydrolysis to another glucose or acceptormolecule. As a side reaction, it is also able to catalyze the isomerization of sucrose to turanose or trehalulose | Synechococcus sp. | ? | - |
? | |
2.4.1.4 | sucrose + [(1->4)-alpha-D-glucosyl]n | - |
Synechococcus sp. | D-fructose + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.4 | AMS | - |
Synechococcus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.4 | 30 | - |
assay at | Synechococcus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.4 | 7.5 | - |
assay at | Synechococcus sp. |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.4.1.4 | Synechococcus sp. | genes spsA,sppA, frkA and amsA are grouped the Suc cluster, whose expression is increased in response to a salt treatment | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.4 | evolution | the enzyme belongs to the aglycoside-hydrolase family 13. Genes spsA,sppA, frkA and amsA are grouped in a transcriptional unit that is named Suc cluster | Synechococcus sp. |