Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Harada, J.; Miyago, S.; Mizoguchi, T.; Azai, C.; Inoue, K.; Tamiaki, H.; Oh-oka, H.
    Accumulation of chlorophyllous pigments esterified with the geranylgeranyl group and photosynthetic competence in the CT2256-deleted mutant of the green sulfur bacterium Chlorobium tepidum (2008), Photochem. Photobiol. Sci., 7, 1179-1187.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.111 gene CT2256, recombinant expression in Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene and partial complementation Chlorobaculum tepidum

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.111 additional information generation of a gene CT2256 deletion mutant, which almost shows no apparent phenotype compared to the wild-type. The purple bacterium Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene is partially complemented with the CT2256 gene, bacteriochlorophyllide a is synthesized in the mutant in addition to accumulating other intermediates, phenotype, overview Chlorobaculum tepidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.111 additional information
-
 additional information steady-state kinetics Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.111 bacteriochlorophyll a + 3 NADP+ Chlorobaculum tepidum
-
 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
1.3.1.111 bacteriochlorophyll a + 3 NADP+ Chlorobaculum tepidum WT2321
-
 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.111 Chlorobaculum tepidum Q8KAB0 gene CT2256
-
1.3.1.111 Chlorobaculum tepidum WT2321 Q8KAB0 gene CT2256
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.111 bacteriochlorophyll a + 3 NADP+
-
 Chlorobaculum tepidum geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
1.3.1.111 bacteriochlorophyll a + 3 NADP+
-
 Chlorobaculum tepidum WT2321 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.111 8
-
 assay at Chlorobaculum tepidum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.111 NADP+
-
 Chlorobaculum tepidum

General Information

EC Number General Information Comment Organism
1.3.1.111 malfunction the CT2256-deleted mutant shows accumulation of chlorophyllous pigments esterified with the geranylgeranyl group and photosynthetic competence, phenotype, overview Chlorobaculum tepidum
1.3.1.111 physiological function the enzyme is involved esterification of bacteriochlorophyll a with DELTA2,6-phytadienol and phytol, respectively, which is produced by reduction of the geranylgeranyl group at the C-17 propionate residue Chlorobaculum tepidum