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Literature summary extracted from

  • Patel, J.M.; Musa, M.M.; Rodriguez, L.; Sutton, D.A.; Popik, V.V.; Phillips, R.S.
    Mutation of Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase at Trp-110 affects stereoselectivity of aromatic ketone reduction (2014), Org. Biomol. Chem., 12, 5905-5910.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.2
-
 Thermoanaerobacter ethanolicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.2 W110A 99.9% conversion of phenylacetone to 84.1% S-product Thermoanaerobacter ethanolicus
1.1.1.2 W110G 99.9% conversion of phenylacetone to 79% S-product, 95.8% conversion of 1-phenyl-2-butanone to 91.6% S-product, 99.1% conversion of 4-phenyl-2-butanone to 70.5% S-product Thermoanaerobacter ethanolicus
1.1.1.2 W110I 99.9% conversion of phenylacetone to 99.9% S-product, 99.4% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.1% conversion of 4-phenyl-2-butanone to 99.9% S-product Thermoanaerobacter ethanolicus
1.1.1.2 W110L 99.9% conversion of phenylacetone to 99.9% S-product, 98.9% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.2% conversion of 4-phenyl-2-butanone to 99.9% S-product Thermoanaerobacter ethanolicus
1.1.1.2 W110M 99.9% conversion of phenylacetone to 99.9% S-product, 97.3% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.3% conversion of 4-phenyl-2-butanone to 99.9% S-product Thermoanaerobacter ethanolicus
1.1.1.2 W110Q 99.9% conversion of phenylacetone to 99.9% S-product, 83.5% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.1% conversion of 4-phenyl-2-butanone to 99.9% S-product Thermoanaerobacter ethanolicus
1.1.1.2 W110V 99.9% conversion of phenylacetone to 99.9% S-product, 99.2% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.1% conversion of 4-phenyl-2-butanone to 99.9% S-product Thermoanaerobacter ethanolicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Thermoanaerobacter ethanolicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 (R)-1-phenyl-2-propanol + NADP+
-
 Thermoanaerobacter ethanolicus phenylacetone + NADPH + H+
-
 ?
1.1.1.2 (S)-1-phenyl-2-propanol + NADP+
-
 Thermoanaerobacter ethanolicus phenylacetone + NADPH + H+
-
 r
1.1.1.2 1-phenyl-2-butanone + NADPH + H+
-
 Thermoanaerobacter ethanolicus (S)-1-phenyl-2-butanol + NADP+
-
 ?
1.1.1.2 4-phenyl-2-butanone + NADPH + H+
-
 Thermoanaerobacter ethanolicus (S)-4-phenyl-2-butanol + NADP+
-
 ?
1.1.1.2 phenylacetone + NADPH + H+
-
 Thermoanaerobacter ethanolicus (S)-1-phenyl-2-propanol + NADP+
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.2 0.008
-
 (R)-1-phenyl-2-propanol mutant W110L, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.025
-
 (R)-1-phenyl-2-propanol mutant W110Q, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.045
-
 (R)-1-phenyl-2-propanol mutant W110M, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.46
-
 (R)-1-phenyl-2-propanol mutant W110I, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.56
-
 (R)-1-phenyl-2-propanol mutant W110A, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.65
-
 (S)-1-phenyl-2-propanol mutant W110L, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 1.2
-
 (R)-1-phenyl-2-propanol mutant W110V, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 1.4
-
 (R)-1-phenyl-2-propanol mutant W110G, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 2.3
-
 (S)-1-phenyl-2-propanol mutant W110Q, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 4.5
-
 (S)-1-phenyl-2-propanol mutant W110M, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 17.8
-
 (S)-1-phenyl-2-propanol mutant W110G, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 18.8
-
 (S)-1-phenyl-2-propanol mutant W110I, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 31.1
-
 (S)-1-phenyl-2-propanol mutant W110A, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 38.6
-
 (S)-1-phenyl-2-propanol mutant W110V, pH 8.9, 50°C Thermoanaerobacter ethanolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADP+
-
 Thermoanaerobacter ethanolicus
1.1.1.2 NADPH
-
 Thermoanaerobacter ethanolicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.2 0.0069
-
 (R)-1-phenyl-2-propanol mutant W110Q, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.024
-
 (R)-1-phenyl-2-propanol mutant W110L, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.121
-
 (R)-1-phenyl-2-propanol mutant W110M, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.188
-
 (R)-1-phenyl-2-propanol mutant W110I, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.284
-
 (R)-1-phenyl-2-propanol mutant W110A, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.336
-
 (R)-1-phenyl-2-propanol mutant W110V, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.551
-
 (S)-1-phenyl-2-propanol mutant W110Q, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 0.639
-
 (R)-1-phenyl-2-propanol mutant W110G, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 1.99
-
 (S)-1-phenyl-2-propanol mutant W110M, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 2.51
-
 (S)-1-phenyl-2-propanol mutant W110L, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 4.935
-
 (S)-1-phenyl-2-propanol mutant W110A, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 5.8
-
 (S)-1-phenyl-2-propanol mutant W110G, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 15.2
-
 (S)-1-phenyl-2-propanol mutant W110I, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.1.1.2 45.3
-
 (S)-1-phenyl-2-propanol mutant W110V, pH 8.9, 50°C Thermoanaerobacter ethanolicus