Literature summary extracted from
Morgan, J.L.W.; Strumillo, J.; Zimmer, J.
Crystallographic snapshot of cellulose synthesis and membrane translocation (2013), Nature, 493, 181-186.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.4.1.12 |
cyclic-di-GMP |
BcsA contains a PilZ domain within its C-terminal, intracellular domain and its activity is strongly stimulated by the bacterial secondary messenger cyclic-di-GMP |
Cereibacter sphaeroides |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.4.1.12 |
recombinant expression of His-tagged subunits BcsA and B in Escherichia coli strain C43 |
Cereibacter sphaeroides |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.4.1.12 |
purified recombinant native and selenomethionine -labeled complex of BcsA and BcsB containing a translocating polysaccharide, from 30% PEG 200, 0.1 M MES, pH 6.5, and 50 mM NaCl at 4°C, 7 days, X-ray diffraction structure determination and analysis, modeling, overview |
Cereibacter sphaeroides |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.4.1.12 |
inner membrane |
cellulose synthases are membrane-embedded glycosyltransferases. BcsB is a periplasmic protein that is anchored to the inner membrane via a single, C-terminal transmembrane helix. BcsA and BcsB are fused |
Cereibacter sphaeroides |
- |
- |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.4.1.12 |
UDP-glucose + [(1->4)-beta-D-glucosyl]n |
Cereibacter sphaeroides |
- |
UDP + [(1->4)-beta-D-glucosyl]n+1 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.1.12 |
Cereibacter sphaeroides |
Q3J125 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.4.1.12 |
recombinant His-tagged native and selenomethionine -labeled subunits BcsA and B from Escherichia coli strain C43 by nickel affinity chromatography and gel filtration |
Cereibacter sphaeroides |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.4.1.12 |
UDP-glucose + [(1->4)-beta-D-glucosyl]n |
- |
Cereibacter sphaeroides |
UDP + [(1->4)-beta-D-glucosyl]n+1 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.4.1.12 |
dimer |
BcsA and BcsB form a 1:1 stoichiometric complex spanning approximately 150 A perpendicular and 55 A parallel to the membrane. The complex is divided into a cuboid-shaped membrane-spanning region sandwiched between large cytoplasmic and periplasmic domains. BcsA contains four N-terminal and four C-terminal transmembrane-helices separated by a large intracellular loop (4/5-loop) that forms a GT-domain (aa 128 to 368). transmembrane domains 3-8 form a narrow channel for the translocating polysaccharide and BcsA's intracellular C-terminus (aa 575 to 759) contains a 6-stranded beta-barrel and a highly curved alpha-helical region that attaches the beta-barrel to the GT-domain. BcsB is a dome-shaped, beta-strand rich, periplasmic protein. Its N-terminal region forms the tip of the dome, whereas the C-terminal transmembrane-anchor interacts with BcsA. Two amphipathic helices further stabilize its interaction with BcsA and the periplasmic water-membrane interface. Domain structures. Modeling, overview |
Cereibacter sphaeroides |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.4.1.12 |
BcsA-B |
- |
Cereibacter sphaeroides |
2.4.1.12 |
inner membrane-associated bacterial cellulose synthase |
- |
Cereibacter sphaeroides |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.4.1.12 |
additional information |
structure of the BcsA-B translocation intermediate revealing the architecture of the cellulose synthase. Subunit BcsA forms a cellulose-conducting channel, modeling for the coupling of cellulose synthesis and translocation in which the nascent polysaccharide is extended by one glucose molecule at a time, overview |
Cereibacter sphaeroides |
2.4.1.12 |
physiological function |
cellulose synthases (CESAs) are membrane-embedded glycosyltransferases, which utilize UDP-activated glucose (UDP-Glc) to processively elongate the nascent polysaccharide in a reaction that inverts the configuration at the anomeric carbon of the newly added sugar from alpha to beta. Cellulose synthesis and transport across the inner bacterial membrane is mediated by a complex of the multi-spanning catalytic BcsA subunit and the membrane-anchored, periplasmic BcsB protein. Structure-function analysis and modeling, overview |
Cereibacter sphaeroides |