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Literature summary extracted from

  • Triboulet, S.; Bougault, C.M.; Laguri, C.; Hugonnet, J.E.; Arthur, M.; Simorre, J.P.
    Acyl acceptor recognition by Enterococcus faecium L,D-transpeptidase Ldtfm (2015), Mol. Microbiol., 98, 90-100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.12 expressed in Escherichia coli BL21(DE3) cells Enterococcus faecium

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.12 K372A the mutant shows 7% of wild type activity Enterococcus faecium
2.3.2.12 K372A/R437A the mutant shows 2% of wild type activity Enterococcus faecium
2.3.2.12 R437A the mutant shows 26% of wild type activity Enterococcus faecium
2.3.2.12 W425A the mutant shows 0.04% of wild type activity Enterococcus faecium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.12 ertapenem
-
 Enterococcus faecium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.12 peptidyl-tRNA1 + aminoacyl-tRNA2 Enterococcus faecium the enzyme is highly specific for acyl donors containing a stem tetrapeptide ending in L-Lys3-D-Ala4 and for acyl acceptors containing a D-iAsn substituted L-Lys3 at the third position of the stem peptide tRNA1 + peptidyl(aminoacyl-tRNA2)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.12 Enterococcus faecium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.12 peptidyl-tRNA1 + aminoacyl-tRNA2 the enzyme is highly specific for acyl donors containing a stem tetrapeptide ending in L-Lys3-D-Ala4 and for acyl acceptors containing a D-iAsn substituted L-Lys3 at the third position of the stem peptide Enterococcus faecium tRNA1 + peptidyl(aminoacyl-tRNA2)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.12 L,D-transpeptidase
-
 Enterococcus faecium
2.3.2.12 Ldtfm
-
 Enterococcus faecium