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Literature summary extracted from

  • Seibold, G.M.; Eikmanns, B.J.
    The glgX gene product of Corynebacterium glutamicum is required for glycogen degradation and for fast adaptation to hyperosmotic stress (2007), Microbiology, 153, 2212-2220.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.196 expressed in Escherichia coli ER2566 cells Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.196 110000
-
 4 * 110000, His6-tagged enzyme, SDS-PAGE Corynebacterium glutamicum
3.2.1.196 417000
-
 native enzyme, gel filtration Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.196 phosphorylase-limit dextrin Corynebacterium glutamicum
-
 maltodextrin + ?
-
 ?
3.2.1.196 phosphorylase-limit dextrin Corynebacterium glutamicum ATCC 13032
-
 maltodextrin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.196 Corynebacterium glutamicum
-
-
-
3.2.1.196 Corynebacterium glutamicum ATCC 13032
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.196 HisTrap column chromatography and Superdex 200 gel filtration Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.196 amylopectin + H2O
-
 Corynebacterium glutamicum maltose
-
 ?
3.2.1.196 amylopectin + H2O
-
 Corynebacterium glutamicum ATCC 13032 maltose
-
 ?
3.2.1.196 glycogen + H2O
-
 Corynebacterium glutamicum maltose + ?
-
 ?
3.2.1.196 glycogen + H2O
-
 Corynebacterium glutamicum ATCC 13032 maltose + ?
-
 ?
3.2.1.196 phosphorylase-limit dextrin
-
 Corynebacterium glutamicum maltodextrin + ?
-
 ?
3.2.1.196 phosphorylase-limit dextrin
-
 Corynebacterium glutamicum ATCC 13032 maltodextrin + ?
-
 ?
3.2.1.196 soluble starch + H2O
-
 Corynebacterium glutamicum maltose + ?
-
 ?
3.2.1.196 soluble starch + H2O
-
 Corynebacterium glutamicum ATCC 13032 maltose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.196 homotetramer 4 * 110000, His6-tagged enzyme, SDS-PAGE Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.196 GlgX
-
 Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.196 95
-
 the His6-tagged enzyme is inactivated by incubation at 95°C for 10 min Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
3.2.1.196 malfunction inactivation of glgX leads to slower growth and to a higher intracellular glycogen pool throughout growth, when compared to those in the parental strain Corynebacterium glutamicum
3.2.1.196 physiological function the enzyme is required for glycogen degradation and for fast adaptation to hyperosmotic stress Corynebacterium glutamicum