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Literature summary extracted from

  • Krutsakorn, B.; Imagawa, T.; Honda, K.; Okano, K.; Ohtake, H.
    Construction of an in vitro bypassed pyruvate decarboxylation pathway using thermostable enzyme modules and its application to N-acetylglutamate production (2013), Microb. Cell Fact., 12, 91.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.1.10 synthesis construction of a bypassed pyruvate decarboxylation pathway, through which pyruvate can be converted to acetyl-CoA, by using a coupled enzyme system consisting of pyruvate decarboxylase from Acetobacter pasteurianus and the CoA-acylating aldehyde dehydrogenase from Thermus thermophilus. A cofactor-balanced and CoA-recycling synthetic pathway for N-acetylglutamate production is designed by coupling the bypassed pathway with the glutamate dehydrogenase from Thermus thermophilus and N-acetylglutamate synthase from Thermotoga maritima. N-Acetylglutamate can be produced from an equimolar mixture of pyruvate and alpha-ketoglutarate with a molar yield of 55% through the synthetic pathway consisting of a mixture of four recombinant Escherichia coli strains having either one of the thermostable enzymes. The overall recycling number of CoA is 27 Thermus thermophilus
4.1.1.1 synthesis construction of a bypassed pyruvate decarboxylation pathway, through which pyruvate can be converted to acetyl-CoA, by using a coupled enzyme system consisting of pyruvate decarboxylase from Acetobacter pasteurianus and the CoA-acylating aldehyde dehydrogenase from Thermus thermophilus. A cofactor-balanced and CoA-recycling synthetic pathway for N-acetylglutamate production is designed by coupling the bypassed pathway with the glutamate dehydrogenase from Thermus thermophilus and N-acetylglutamate synthase from Thermotoga maritima. N-Acetylglutamate can be produced from an equimolar mixture of pyruvate and alpha-ketoglutarate with a molar yield of 55% through the synthetic pathway consisting of a mixture of four recombinant Escherichia coli strains having either one of the thermostable enzymes. The overall recycling number of CoA is 27 Acetobacter pasteurianus
4.1.1.1 synthesis construction of a bypassed pyruvate decarboxylation pathway, through which pyruvate can be converted to acetyl-CoA. The coupled enzyme system consists of pyruvate decarboxylase from Acetobacter pasteurianus and the CoA-acylating aldehyde dehydrogenase from Thermus thermophilus. A cofactor-balanced and CoA-recycling synthetic pathway for N-acetylglutamate production is established by coupling the bypassed pathway with the glutamate dehydrogenase from Thermus thermophilus and N-acetylglutamate synthase from Thermotoga maritima. N-Acetylglutamate can be produced from an equimolar mixture of pyruvate and alpha-ketoglutarate with a molar yield of 55% through the synthetic pathway consisting of a mixture of four recombinant Escherichia coli strains having either one of the thermostable enzymes. The overall recycling number of CoA is 27 Acetobacter pasteurianus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.10 expression in Escherichia coli Thermus thermophilus
4.1.1.1 expression in Escherichia coli Acetobacter pasteurianus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.10 Thermus thermophilus Q53WH9
-
-
1.2.1.10 Thermus thermophilus DSM 579 Q53WH9
-
-
4.1.1.1 Acetobacter pasteurianus C7JF72
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.1 0.025
-
 50°C, pH 8.5 Acetobacter pasteurianus
4.1.1.1 0.046
-
 50°C, pH 6.0 Acetobacter pasteurianus
4.1.1.1 0.046
-
 50°C, pH 8.0 Acetobacter pasteurianus
4.1.1.1 0.058
-
 50°C, pH 7.5 Acetobacter pasteurianus
4.1.1.1 0.066
-
 50°C, pH 6.5 Acetobacter pasteurianus
4.1.1.1 0.078
-
 50°C, pH 7.0 Acetobacter pasteurianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.1 pyruvate + CoA + NAD+
-
 Acetobacter pasteurianus acetyl-CoA + CO2 + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.10 TTHB247
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.1 NAD+
-
 Acetobacter pasteurianus