Literature summary extracted from

Chen, X.; Xu, J.; Yang, L.; Yuan, Z.; Xiao, S.; Zhang, Y.; Liang, C.; He, M.; Guo, Y.
Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli (2015), J. Ind. Microbiol. Biotechnol., 42, 1473-1479.

Application

EC Number	Application	Comment	Organism
4.1.1.1	synthesis	expression of branched-chain alpha-oxo acid decarboxylase from Lactococcus lactis subsp. lactis KivD and alcohol dehydrogenase from Zymomonas mobilis AdhB in Escherichia coli for higher alcohol production. In LB medium, the resulting strain produces much more 3-methyl-1-butanol (104 mg/l) than isobutanol (24 mg/l). In 5 g/l glucose-containing medium, the production of two alcohols is similar, 156 and 161 mg/l for C4 (isobutanol) and C5 (3-methyl-1-butanol) alcohol, respectively. The increase of glucose content and the adding of alpha-keto acids facilitate the production of C4 and C5 alcohols. The enzyme activities of pure Kivd on alpha-ketoisovalerate and alpha-ketoisocaproate are 26.77 and 21.24 micromol/min and mg, respectively	Lactococcus lactis subsp. lactis

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.1.1.1	-	Lactococcus lactis subsp. lactis

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.1.1	Lactococcus lactis subsp. lactis	-	-	-
4.1.1.1	Lactococcus lactis subsp. lactis CICC 6246	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.1.1.1	21.24	-	substrate 2-oxoisocaproate, pH 6.0, 37°C	Lactococcus lactis subsp. lactis
4.1.1.1	26.77	-	substrate 2-oxoisovalerate, pH 6.0, 37°C	Lactococcus lactis subsp. lactis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.1.1	2-oxoisocaproate	-	Lactococcus lactis subsp. lactis	3-methylbutanal + CO2	-	?
4.1.1.1	2-oxoisocaproate	-	Lactococcus lactis subsp. lactis CICC 6246	3-methylbutanal + CO2	-	?
4.1.1.1	2-oxoisovalerate	-	Lactococcus lactis subsp. lactis	2-methylpropanal + CO2	-	?
4.1.1.1	2-oxoisovalerate	-	Lactococcus lactis subsp. lactis CICC 6246	2-methylpropanal + CO2	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.1.1.1	KivD	-	Lactococcus lactis subsp. lactis

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Release 2023.1 (January 2023)