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Literature summary extracted from

  • Rodriguez, A.C.; Yu, S.H.; Li, B.; Zegzouti, H.; Kohler, J.J.
    Enhanced transfer of a photocross-linking N-acetylglucosamine (GlcNAc) analog by an O-GlcNAc transferase mutant with converted substrate specificity (2015), J. Biol. Chem., 290, 22638-22648.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.255 analysis metabolic labeling method to introduce the diazirine photocross-linking functional group onto O-GlcNAc residues in mammalian cells. Cells are engineered to produce diazirine-modified UDP-GlcNAc (UDP-Glc-NDAz), which is transferred to substrate proteins by endogenous OGT, producing O-GlcNDAz. Modified proteins can be covalently cross-linked to their binding partners, providing information about O-GlcNAc-dependent interactions Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.255 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.255 C917A mutant efficiently transfers diazirine-modified GlcNDAz and has altered substrate specificity, preferring to transfer GlcNDAz rather than GlcNAc to protein substrates Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.255 0.0021
-
 UDP-GlcNAc wild-type, pH 7.2 22°C Homo sapiens
2.4.1.255 0.0047
-
 UDP-N-azidoacetylglucosamine wild-type, pH 7.2 22°C Homo sapiens
2.4.1.255 0.0085
-
 UDP-N-azidoacetylglucosamine mutant C917A, pH 7.2 22°C Homo sapiens
2.4.1.255 0.0155
-
 UDP-GlcNAc mutant C917A, pH 7.2 22°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.255 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.255 casein kinase II + UDP-GlcNAc
-
 Homo sapiens ? + UDP
-
 ?
2.4.1.255 casein kinase II + UDP-N-azidoacetylglucosamine
-
 Homo sapiens ? + UDP
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.255 0.0015
-
 UDP-N-azidoacetylglucosamine wild-type, pH 7.2 22°C Homo sapiens
2.4.1.255 0.0027
-
 UDP-GlcNAc mutant C917A, pH 7.2 22°C Homo sapiens
2.4.1.255 0.008
-
 UDP-GlcNAc wild-type, pH 7.2 22°C Homo sapiens
2.4.1.255 0.0085
-
 UDP-N-azidoacetylglucosamine mutant C917A, pH 7.2 22°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.255 0.001
-
 UDP-N-azidoacetylglucosamine mutant C917A, pH 7.2 22°C Homo sapiens
2.4.1.255 0.002
-
 UDP-GlcNAc mutant C917A, pH 7.2 22°C Homo sapiens
2.4.1.255 0.003
-
 UDP-N-azidoacetylglucosamine wild-type, pH 7.2 22°C Homo sapiens
2.4.1.255 0.0037
-
 UDP-GlcNAc wild-type, pH 7.2 22°C Homo sapiens