EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.20 | expressed in Saccharomyces cerevisiae mutant H1246 | Linum usitatissimum |
2.3.1.23 | two genes LPCAT, LPCAT1 and LPCAT2, containing eight exons and seven introns, respectively, DNA and amino acid seqquence determination and analysis, co-expression of flax genes LPCAT1 and diacylglycerol acyltransferase DGAT1-1 in the yeast quintuple mutant strain H1246 DELTAale1 significantly increasing 18-carbon polyunsaturated fatty acids in triacylglycerol with a concomitant decrease of 18-carbon polyunsaturated fatty acids in phospholipid. The specific activity of flax LPCAT1-catalyzed forward reaction is about 1000 times higher than that of flax DGAT1-catalyzed reaction when the expressed recombinant enzymes are assessed in yeast microsomes under in vitro enzyme assays | Linum usitatissimum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.23 | microsome | - |
Linum usitatissimum | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.23 | acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | Linum usitatissimum | phosphatidylcholine is the major site for polyunsaturated fatty acid synthesis | CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.20 | Linum usitatissimum | - |
- |
- |
2.3.1.23 | Linum usitatissimum | - |
isozymes LPCAT1 and LPCAT2 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.20 | additional information | co-expressing flax diacylglycerol acyltransferase1-1 (DGAT1-1) and acyl-CoA:lysophosphatidylcholine acyltransferase1 (LPCAT1) in a yeast quintuple mutant significantly increases 18-carbon polyunsaturated fatty acids in triacylglycerol with a concomitant decrease of 18-carbon polyunsaturated fatty acids in phospholipid. The specific activity of overall LPCAT1 and DGAT1-1 coupling process exhibited a preference for transferring 14C-labeled linoleoyl or linolenoyl than oleoyl moieties from the sn-2 position of phosphatidylcholine to triacylglycerol | Linum usitatissimum | ? | - |
? | |
2.3.1.20 | oleoyl-CoA + 1,2-dioleoyl-sn-glycerol | - |
Linum usitatissimum | CoA + trioleoylglycerol | - |
? | |
2.3.1.23 | acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | phosphatidylcholine is the major site for polyunsaturated fatty acid synthesis | Linum usitatissimum | CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
r | |
2.3.1.23 | acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | the reverse reaction of enzyme LPCAT uses phosphatidylcholine as substrate and forms acyl-CoA and lysophosphatidylcholine | Linum usitatissimum | CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.20 | DGAT | - |
Linum usitatissimum |
2.3.1.20 | diacylglycerol acyltransferase | - |
Linum usitatissimum |
2.3.1.23 | acyl-CoA:lysophosphatidylcholine acyltransferase | - |
Linum usitatissimum |
2.3.1.23 | LPCAT | - |
Linum usitatissimum |
2.3.1.23 | LPCAT1 | - |
Linum usitatissimum |
2.3.1.23 | LPCAT2 | - |
Linum usitatissimum |
2.3.1.23 | lysophosphatidylcholine acyltransferase | - |
Linum usitatissimum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.23 | 30 | - |
assay at, forward and reverse reaction | Linum usitatissimum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.23 | 7.2 | - |
assay at, forward and reverse reaction | Linum usitatissimum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.23 | evolution | the LPCAT genes are highly conserved, suggesting that the duplicated LPCAT genes were derived from a segmental duplication event. The segmental duplication is the result of a recent whole-genome duplication event in flax | Linum usitatissimum |
2.3.1.23 | metabolism | the enzyme is involved in the Kennedy pathway for triacylglycerol synthesis, biochemical coupling of isozyme LPCAT1 and the diacylglycerol acyltransferase DGAT1-1-catalyzed reactions, overview. Both enzymes are required for synthesis of triacylgycerol | Linum usitatissimum |
2.3.1.23 | additional information | the specific activity of flax LPCAT1-catalyzed forward reaction is about 1000 times higher than that of flax DGAT1-catalyzed reaction when the coexpressed recombinant enzymes are assessed in yeast microsomes under in vitro enzyme assays, overview | Linum usitatissimum |
2.3.1.23 | physiological function | phosphatidylcholine is the major site for polyunsaturated fatty acid synthesis. Enzyme lysophosphatidylcholine acyltransferase can transfer polyunsaturated fatty acids on phosphatidylcholine directly into the acyl-CoA pool, making these polyunsaturated fatty acids available for the diacylglycerol acyltransferase (DGAT)-catalyzed reaction for triacylglycerol production, proposed mechanism, overview | Linum usitatissimum |