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Literature summary extracted from

  • Yadav, B.; Bhatnagar, S.; Ahmad, M.F.; Jain, P.; Pratyusha, V.A.; Kumar, P.; Komath, S.S.
    First step of glycosylphosphatidylinositol (GPI) biosynthesis cross-talks with ergosterol biosynthesis and Ras signaling in Candida albicans (2014), J. Biol. Chem., 289, 3365-3382.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.198 additional information generation of single knock-out of gene GPI2 and double knockout mutants of genes GPI1 and GPI119 from Cancdida albicans strain BWP17, microsomes generated from GPI2 mutants exhibited lower GPI-GnT activity as compared with CAI4, phenotypes, overview Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.198 cell wall
-
 Candida albicans 5618
-
2.4.1.198 microsome
-
 Candida albicans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.198 Mg2+ required Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.198 UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol Candida albicans
-
 UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.198 Candida albicans Q5A6L6 enzyme complex subunit GPI2; diverse strains
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.198 UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol
-
 Candida albicans UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.198 GPI-GnT
-
 Candida albicans
2.4.1.198 GPI-N-acetylglucosaminyltransferase
-
 Candida albicans
2.4.1.198 GPI2
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.198 30
-
 assay at Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.198 7.5
-
 assay at Candida albicans

General Information

EC Number General Information Comment Organism
2.4.1.198 malfunction GPI19 mutants show up-regulation of GPI2, whereas GPI2 mutants show upregulation of GPI19. GPI2 disruption leads to defective hyphal morphogenesis due to altered Ras signaling. Reintroduction of GPI2 into the GPI2 heterozygous strain can reverse the phenotypes Candida albicans
2.4.1.198 metabolism analysis of the regulatory function of the enzyme, overview Candida albicans
2.4.1.198 physiological function the enzyme complex, GPI-N-acetylglucosaminyltransferase (GPI-GnT), is involved in the first step of GPI anchor biosynthesis in eukaryotes. Candida albicans has several glycosylphosphatidylinositol (GPI)-anchored virulence factors. Inhibiting GPI biosynthesis attenuates its virulence. Enzyme complex subunit GPI2 is essential for growth and hyphal morphogenesis and is needed for filamentation. The GPI-GnT enzyme complex accessory subunits, GPI2 and GPI19, exhibit opposite effects on ergosterol biosynthesis and Ras signaling (which determines hyphal morphogenesis), because the two subunits negatively regulate one another. GPI19 controls ergosterol biosynthesis through ERG11 levels, whereas GPI2 determines the filamentation by cross-talk with Ras1 signaling. GPI2 affects GPI anchor biosynthesis and cell wall Candida albicans