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Literature summary extracted from

  • Morimoto, R.; Shindou, H.; Tarui, M.; Shimizu, T.
    Rapid production of platelet-activating factor is induced by protein kinase Calpha-mediated phosphorylation of lysophosphatidylcholine acyltransferase 2 protein (2014), J. Biol. Chem., 289, 15566-15576.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.67 additional information the enzyme is rapidly phosphorylated after methylcarbamylplatelet-activating factor stimulation to enhance its enzymatic activity. Knockdown of PKCalpha results in significant decrease of lyso-PAFAT activation after ATP stimulation Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.67 stable recombinant enzyme expression in RAW264.7 cells, recombinant expression in CHO-S-PAFR cells Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.67 Ca2+ dependent on Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.67 acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine Mus musculus
-
 CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.67 acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine Mus musculus C57/BL6J
-
 CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.67 additional information Mus musculus LPCAT2 also possesses lysophosphatidylcholine acyltransferase, EC: 2.3.1.23, activity that catalyzes the incorporation of arachidonoyl-CoA into membrane phosphatidylcholine ?
-
 ?
2.3.1.67 additional information Mus musculus C57/BL6J LPCAT2 also possesses lysophosphatidylcholine acyltransferase, EC: 2.3.1.23, activity that catalyzes the incorporation of arachidonoyl-CoA into membrane phosphatidylcholine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.67 Mus musculus Q8BYI6
-
-
2.3.1.67 Mus musculus C57/BL6J Q8BYI6
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.67 phosphoprotein stimulation by either platelet-activating factor or ATP results in PKCalpha-mediated phosphorylation of the enzyme at Ser34 causing augmentation of Vmax with minimal Km change. The enzyme is rapidly phosphorylated after methylcarbamylplatelet-activating factor stimulation to enhance its enzymatic activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.67 acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine
-
 Mus musculus CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.67 acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine
-
 Mus musculus C57/BL6J CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.67 additional information LPCAT2 also possesses lysophosphatidylcholine acyltransferase, EC: 2.3.1.23, activity that catalyzes the incorporation of arachidonoyl-CoA into membrane phosphatidylcholine Mus musculus ?
-
 ?
2.3.1.67 additional information LPCAT2 also possesses lysophosphatidylcholine acyltransferase, EC: 2.3.1.23, activity that catalyzes the incorporation of arachidonoyl-CoA into membrane phosphatidylcholine Mus musculus C57/BL6J ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.67 acetyl-CoA:lyso-PAF acetyltransferase
-
 Mus musculus
2.3.1.67 LPCAT2
-
 Mus musculus
2.3.1.67 lyso-PAFAT
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.67 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.67 7.4
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.67 acetyl-CoA
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.67 metabolism platelet-activating factor is synthesized rapidly in the remodeling pathway from its precursor membrane phospholipid, 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine, involving the enzyme Mus musculus
2.3.1.67 physiological function platelet-activating factor (PAF), a potent proinflammatory lipid mediator, is synthesized rapidly in response to extracellular stimuli by the activation of acetyl-CoA:lyso-PAF acetyltransferase, lyso-PAFAT. The enzyme is rapidly phosphorylated after methylcarbamylplatelet-activating factor stimulation to enhance its enzymatic activity Mus musculus