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Literature summary extracted from

  • Banerjee, A.; Wu, Y.; Banerjee, R.; Li, Y.; Yan, H.; Sharkey, T.D.
    Feedback inhibition of deoxy-D-xylulose-5-phosphate synthase regulates the methylerythritol 4-phosphate pathway (2013), J. Biol. Chem., 288, 16926-16936.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.7 expressed in Escherichia coli BL21(DE3)pLysS cells Populus trichocarpa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.7 dimethylallyl diphosphate competitive inhibitor Populus trichocarpa
2.2.1.7 isopentenyl diphosphate competitive inhibitor Populus trichocarpa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.7 0.0185
-
 D-glyceraldehyde 3-phosphate at pH 8.0 and 37°C Populus trichocarpa
2.2.1.7 0.0878
-
 pyruvate at pH 8.0 and 37°C Populus trichocarpa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.2.1.7 chloroplast
-
 Populus trichocarpa 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.7 Mg2+ required Populus trichocarpa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.7 pyruvate + D-glyceraldehyde 3-phosphate Populus trichocarpa
-
 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.7 Populus trichocarpa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.7 ammonium sulfate precipitation and Ni-NTA column chromatography Populus trichocarpa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.7 pyruvate + D-glyceraldehyde 3-phosphate
-
 Populus trichocarpa 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.7 deoxyxylulose-5-phosphate synthase
-
 Populus trichocarpa
2.2.1.7 DXS
-
 Populus trichocarpa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.7 0.53
-
 pyruvate at pH 8.0 and 37°C Populus trichocarpa
2.2.1.7 0.64
-
 D-glyceraldehyde 3-phosphate at pH 8.0 and 37°C Populus trichocarpa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.7 8
-
-
 Populus trichocarpa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.7 6.5 8.5 the enzyme does not show substantial activity below pH 6.5 and above pH 8.5 Populus trichocarpa

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.7 thiamine diphosphate
-
 Populus trichocarpa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2.1.7 0.0654
-
 isopentenyl diphosphate at pH 8.0 and 37°C Populus trichocarpa
2.2.1.7 0.0813
-
 dimethylallyl diphosphate at pH 8.0 and 37°C Populus trichocarpa