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Literature summary extracted from

  • Manavalan, T.; Manavalan, A.; Thangavelu, K.P.; Heese, K.
    Characterization of a novel endoglucanase from Ganoderma lucidum (2015), J. Basic Microbiol., 55, 761-771.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Cr2+ 1 mM, 27% residual activity Ganoderma lucidum
3.2.1.4 EDTA 1 mM, 17% residual activity Ganoderma lucidum
3.2.1.4 Fe2+ 1 mM, 65% residual activity Ganoderma lucidum
3.2.1.4 Hg2+ 1 mM, 12% residual activity Ganoderma lucidum
3.2.1.4 N-bromosuccinimide 1 mM, 35% residual activity Ganoderma lucidum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ 1 mM, 116% of initial activtiy Ganoderma lucidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 64000
-
 x * 64000, SDS-PAGE Ganoderma lucidum

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.4 Acetone 1%, 81% of initial activity Ganoderma lucidum
3.2.1.4 benzene 1%, 111% of initial activity Ganoderma lucidum
3.2.1.4 Butanol 1%, 49% of initial activity Ganoderma lucidum
3.2.1.4 chloroform 1%, 106% of initial activity Ganoderma lucidum
3.2.1.4 hexane 1%, 115% of initial activity Ganoderma lucidum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Ganoderma lucidum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Ganoderma lucidum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 787
-
 pH 5.0, 35°C Ganoderma lucidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O 12% of the activity with carboxymethyl cellulose Ganoderma lucidum ?
-
 ?
3.2.1.4 birchwood xylan + H2O 2% of the activity with carboxymethyl cellulose Ganoderma lucidum ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Ganoderma lucidum ?
-
 ?
3.2.1.4 cellobiose + H2O 25% of the activity with carboxymethyl cellulose Ganoderma lucidum ?
-
 ?
3.2.1.4 laminarin + H2O 5% of the activity with carboxymethyl cellulose Ganoderma lucidum ?
-
 ?
3.2.1.4 lichenan + H2O 77% of the activity with carboxymethyl cellulose Ganoderma lucidum ?
-
 ?
3.2.1.4 additional information no substrates: chitosan, amylose Ganoderma lucidum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 64000, SDS-PAGE Ganoderma lucidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 35
-
-
 Ganoderma lucidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 30 60 stable Ganoderma lucidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
-
 Ganoderma lucidum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 4 7 stable Ganoderma lucidum

Expression

EC Number Organism Comment Expression
3.2.1.4 Ganoderma lucidum expression upon supplementation of medium with 1% (w/v) wheat bran additional information