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Literature summary extracted from

  • Anonsen, J.H.; Vik, A.; Borud, B.; Viburiene, R.; Aas, F.E.; Kidd, S.W.; Aspholm, M.; Koomey, M.
    Characterization of a unique tetrasaccharide and distinct glycoproteome in the O-linked protein glycosylation system of Neisseria elongata subsp. glycolytica (2016), J. Bacteriol., 198, 256-267.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.36 gene pglB, DNA and amino acid sequence determination, genetic organization, comparative pgl gene content and synteny between Neisseria elongata subsp. glycolytica strain ATCC 29315 and Neisseria gonorrhoeae strain FA1090. The core pgl locus of Neisseria elongata subsp. glycolytica encompasses pglG, pglH, pglC, and a split (two-ORF) version of pglB, whereas pglF and pglD are localized elsewhere in the genome. The pglO gene is also localized outside the core locus in Neisseria elongata subsp. glycolytica, while the pglA, pglE, and pglI genes are not found in Neisseria elongata subsp. glycolytica Neisseria elongata subsp. glycolytica
2.7.8.36 gene pglB, DNA and amino acid sequence determination, genetic organization, comparative pgl gene content and synteny between Neisseria elongata subsp. glycolytica strain ATCC 29315 and Neisseria gonorrhoeae strain FA1090. The pglO gene is localized outside the core locus in Neisseria gonorrhoeae Neisseria gonorrhoeae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.36 UDP-N,N'-diacetylbacillosamine + tritrans,heptacis-undecaprenyl phosphate Neisseria gonorrhoeae
-
 UMP + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
-
 ?
2.7.8.36 UDP-N,N'-diacetylbacillosamine + tritrans,heptacis-undecaprenyl phosphate Neisseria elongata subsp. glycolytica
-
 UMP + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
-
 ?
2.7.8.36 UDP-N,N'-diacetylbacillosamine + tritrans,heptacis-undecaprenyl phosphate Neisseria gonorrhoeae FA 1090
-
 UMP + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
-
 ?
2.7.8.36 UDP-N,N'-diacetylbacillosamine + tritrans,heptacis-undecaprenyl phosphate Neisseria elongata subsp. glycolytica ATCC 29315
-
 UMP + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B69 Neisseria elongata subsp. glycolytica A0A0N7IL44
-
-
2.7.8.36 Neisseria elongata subsp. glycolytica
-
-
-
2.7.8.36 Neisseria elongata subsp. glycolytica ATCC 29315
-
-
-
2.7.8.36 Neisseria gonorrhoeae
-
-
-
2.7.8.36 Neisseria gonorrhoeae FA 1090
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B69 UDP-D-glucose + diNAcBac-PP-tritrans,heptacis-undecaprenol
-
 Neisseria elongata subsp. glycolytica glucosyl-diNAcBac-PP-tritrans,heptacis-undecaprenol
-
 ?
2.7.8.36 UDP-N,N'-diacetylbacillosamine + tritrans,heptacis-undecaprenyl phosphate
-
 Neisseria gonorrhoeae UMP + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
-
 ?
2.7.8.36 UDP-N,N'-diacetylbacillosamine + tritrans,heptacis-undecaprenyl phosphate
-
 Neisseria elongata subsp. glycolytica UMP + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
-
 ?
2.7.8.36 UDP-N,N'-diacetylbacillosamine + tritrans,heptacis-undecaprenyl phosphate
-
 Neisseria gonorrhoeae FA 1090 UMP + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
-
 ?
2.7.8.36 UDP-N,N'-diacetylbacillosamine + tritrans,heptacis-undecaprenyl phosphate
-
 Neisseria elongata subsp. glycolytica ATCC 29315 UMP + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B69 PglH
-
 Neisseria elongata subsp. glycolytica
2.7.8.36 pclB
-
 Neisseria gonorrhoeae
2.7.8.36 pclB
-
 Neisseria elongata subsp. glycolytica

General Information

EC Number General Information Comment Organism
2.4.1.B69 physiological function a null mutation in PglH leads to the expression of a diNAcBac monosaccharide glycoform. PglH protein participates in the synthesis of Und-PP-diNAcBac-Glc (i.e. undecaprenyl diphosphate-4-glyceramido-2-acetamido-2,4,6-trideoxy-alpha-D-hexose) similarly to its gonococcal and meningococcal orthologues Neisseria elongata subsp. glycolytica
2.7.8.36 metabolism the enzyme is part of the broad-spectrum O-linked glycosylation pathway expressed by neisserial species, overview. PglB, -C, and -D are required for the synthesis of UDP-N'-diacetylbacillosamine (UDP-diNAcBac) and transfer of the phospho-diNAcBac to undecaprenyl phosphate. This glycoform can be further modified by the glycosyltransferases PglA and PglH/PglH2. The PglE glycosyltransferase can modify only the disaccharide glycoform generated by PglA Neisseria gonorrhoeae
2.7.8.36 metabolism the enzyme is part of the broad-spectrum O-linked glycosylation pathway expressed by neisserial species, overview. PglB, -C, and -D are required for the synthesis of UDP-N'-diacetylbacillosamine (UDP-diNAcBac) and transfer of the phospho-diNAcBac to undecaprenyl phosphate. This glycoform can be further modified by the glycosyltransferases PglA and PglH/PglH2. The PglE glycosyltransferase can modify only the disaccharide glycoform generated by PglA Neisseria elongata subsp. glycolytica
2.7.8.36 physiological function the PglD and PglC proteins carry out NAD+-dependent dehydratase and aminotransferase reactions, respectively, to convert UDP-GlcNAc to UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha--D-glucose. PglB then acts as a bifunctional enzyme, which catalyzes the amino acetylation of UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucose to form UDP-di-N-acetylbacillosamine (diNAcBac) and the transfer of the phospho-sugar to undecaprenyl phosphate Neisseria gonorrhoeae
2.7.8.36 physiological function the PglD and PglC proteins carry out NAD+-dependent dehydratase and aminotransferase reactions, respectively, to convert UDP-GlcNAc to UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucose. PglB then acts as a bifunctional enzyme, which catalyzes the amino acetylation of UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucose to form UDP-di-N-acetylbacillosamine (diNAcBac) and the transfer of the phospho-sugar to undecaprenyl phosphate Neisseria elongata subsp. glycolytica