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Literature summary extracted from

  • Doi, Y.; Ikegami, Y.
    Pyruvate formate-lyase is essential for fumarate-independent anaerobic glycerol utilization in the Enterococcus faecalis strain W11 (2014), J. Bacteriol., 196, 2472-2480.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.54 gene pflB, enzyme expression analysis Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.54 additional information construction of a pflB gene disruptant of strain W11 that barely utilizes glycerol under anaerobic conditions, but the growth of the DELTApfl mutant strain cultured with either glucose or dihydroxyacetone under anaerobic conditions is the same as that of wild-type strain W11 Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 acetyl-CoA + formate Enterococcus faecalis
-
 CoA + pyruvate
-
 ?
2.3.1.54 acetyl-CoA + formate Enterococcus faecalis W11
-
 CoA + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.54 Enterococcus faecalis
-
 gene pflB
-
2.3.1.54 Enterococcus faecalis W11
-
 gene pflB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.54 acetyl-CoA + formate
-
 Enterococcus faecalis CoA + pyruvate
-
 ?
2.3.1.54 acetyl-CoA + formate
-
 Enterococcus faecalis W11 CoA + pyruvate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.54 PFL
-
 Enterococcus faecalis
2.3.1.54 pyruvate formate-lyase
-
 Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.54 acetyl-CoA
-
 Enterococcus faecalis

Expression

EC Number Organism Comment Expression
2.3.1.54 Enterococcus faecalis expression of pyruvate formate-lyase is upregulated during anaerobic culture with glycerol up

General Information

EC Number General Information Comment Organism
2.3.1.54 metabolism NADH generated from anaerobic glycerol metabolism in the absence of fumarate is oxidized through the pyruvate formate-lyase-ethanol fermentation pathway. Thus, the enzyme is essential to avoid the accumulation of excess NADH during fumarate-independent anaerobic glycerol metabolism Enterococcus faecalis