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Literature summary extracted from

  • Lowry, B.; Robbins, T.; Weng, C.H.; OBrien, R.V.; Cane, D.E.; Khosla, C.
    In vitro reconstitution and analysis of the 6-deoxyerythronolide B synthase (2013), J. Am. Chem. Soc., 135, 16809-16812.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.94 analysis kinetic assay for the megasynthase that stoichiometrically relates NADPH consumption to polyketide production Saccharopolyspora erythraea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.94 expression in Escherichia coli Saccharopolyspora erythraea

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.94 additional information development of expression and purification protocols for subunits DEBS2 and DEBS3 from recombinant strains of Escherichia coli Saccharopolyspora erythraea

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.94 Saccharopolyspora erythraea Q03131 and Q03132 and Q03133 Q03131, i.e. modules 1 and 2, Q03132, i.e. modules 3 and 4, Q03133, i.e. modules 5 and 6
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.94 propanoyl-CoA + 5 (2S)-methylmalonyl-CoA + (2S)-ethylmalonyl-CoA + 6 NADPH + 6 H+
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 Saccharopolyspora erythraea (3R,4S,5R,6R)-6-ethyl-4-hydroxy-3,5-dimethyltetrahydro-2H-pyran-2-one + ? product of truncated enzyme consisting of loading didomain-module1-module2+thioesterase ?
2.3.1.94 propanoyl-CoA + 5 (2S)-methylmalonyl-CoA + (2S)-ethylmalonyl-CoA + 6 NADPH + 6 H+
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 Saccharopolyspora erythraea (3R,5R,6S)-6-[(2S,3R)-3-hydroxypentan-2-yl]-3,5-dimethyldihydro-2H-pyran-2,4(3H)-dione + ? product of truncated enzyme consisting of loading didomain-module1-module2-module3+thioesterase ?
2.3.1.94 propanoyl-CoA + 5 (2S)-methylmalonyl-CoA + (2S)-ethylmalonyl-CoA + 6 NADPH + 6 H+
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 Saccharopolyspora erythraea 8-ethyl-8-desmethyl-6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+ putative analog, 8-ethyl-8-desmethyl-6-deoxyerythronolide B, is generated by the assembly line in the presence of non-limiting concentrations of ethylmalonyl-CoA and is produced in comparable amounts to the natural 6-dEB product ?
2.3.1.94 propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+
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 Saccharopolyspora erythraea 6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.94 DEBS
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 Saccharopolyspora erythraea