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Literature summary extracted from

  • Shrimal, S.; Gilmore, R.
    Reduced expression of the oligosaccharyltransferase exacerbates protein hypoglycosylation in cells lacking the fully assembled oligosaccharide donor (2015), Glycobiology, 25, 774-783.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.267 Cricetulus griseus G3GZD6
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2.4.1.267 Homo sapiens Q9Y672
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.267 fibroblast
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 Homo sapiens
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2.4.1.267 MI8-5 CHO cell CHO cell line Cricetulus griseus
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General Information

EC Number General Information Comment Organism
2.4.1.267 physiological function ALG6 deficient MI8-5 cells express 2fold lower levels of oligosaccharyltransferase STT3B than the parental Chinese hamster ovary cells. The combination of reduced expression of STT3B and the lack of the optimal Dol-PP-GlcNAc2Man9Glc3 donor synergize to cause very severe hypoglycosylation of proteins in MI8-5 cells Cricetulus griseus
2.4.1.267 physiological function fibroblasts from an ALG6-congenital disorders of glycosylation patient that carries the A333V mutation on the maternal ALG6 allele and the S308R and Y131H mutations on the paternal ALG6 allele assemble Dol-PP-GlcNAc2Man9 as the largest oligosaccharide donor. 30–40% of oligosaccharyltransferase STT3A-dependent glycosylation sites and 20% of oligosaccharyltransferase STT3B-dependent sites are skipped in ALG6-congenital disorders of glycosylation fibroblasts Homo sapiens