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Literature summary extracted from

  • Shi, Q.; Chung, J.S.
    Trehalose metabolism in the blue crab Callinectes sapidus: isolation of multiple structural cDNA isoforms of trehalose-6-phosphate synthase and their expression in muscles (2014), Gene, 536, 105-113.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.15 isozyme TPS1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crabl. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis Callinectes sapidus
2.4.1.15 isozyme TPS1a, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis Callinectes sapidus
2.4.1.15 isozyme TPS1b, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis Callinectes sapidus
2.4.1.15 isozyme TPS1c, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis Callinectes sapidus
2.4.1.216 DNA and amino acid sequence determination and analysis, sequence comparison, quantitative RT-PCR enzyme expression analysis Callinectes sapidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.15 UDP-glucose + D-glucose 6-phosphate Callinectes sapidus
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.216 alpha,alpha-trehalose 6-phosphate + phosphate Callinectes sapidus
-
 D-glucose 6-phosphate + beta-D-glucose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Callinectes sapidus E1U2Z7 isozyme 1; collected from the upper Chesapeake Bay, China
-
2.4.1.15 Callinectes sapidus E1U2Z8 isozyme 1a; collected from the upper Chesapeake Bay, China
-
2.4.1.15 Callinectes sapidus E1U2Z9 isozyme 1b; collected from the upper Chesapeake Bay, China
-
2.4.1.15 Callinectes sapidus E1U300 isozyme 1c; collected from the upper Chesapeake Bay, China
-
2.4.1.216 Callinectes sapidus
-
 collected from the upper Chesapeake Bay, China
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.15 basal swimming muscle
-
 Callinectes sapidus
-
2.4.1.15 claw muscle in both males and females, the darker muscles exhibit greater amounts of trehalose, trehalose 6-phosphate synthase, and trehalase activities than the light colored Callinectes sapidus
-
2.4.1.15 additional information ubiquitous expression of CasTPS in most tissues Callinectes sapidus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 UDP-glucose + D-glucose 6-phosphate
-
 Callinectes sapidus UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.216 alpha,alpha-trehalose 6-phosphate + phosphate
-
 Callinectes sapidus D-glucose 6-phosphate + beta-D-glucose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.15 More isozyme domain structure, overview Callinectes sapidus
2.4.1.15 More isozyme domain structure, overview. The isozyme 1 encodes both a putative TPS and a TPP domain in tandem Callinectes sapidus
2.4.1.15 More isozyme domain structure, overview. The isozyme 1b encodes both a putative TPS and a TPP domain in tandem Callinectes sapidus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.15 TPS
-
 Callinectes sapidus
2.4.1.15 trehalose-6-phosphate synthase
-
 Callinectes sapidus
2.4.1.216 TPP
-
 Callinectes sapidus
2.4.1.216 trehalose-6-phosphate phosphatase
-
 Callinectes sapidus

General Information

EC Number General Information Comment Organism
2.4.1.15 additional information differences in muscle trehalose content in female and male animals due to polymorphisms Callinectes sapidus