Literature summary extracted from
Zhang, Y.; Dai, Z.; Krivoruchko, A.; Chen, Y.; Siewers, V.; Nielsen, J.
Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth (2015), FEMS Yeast Res., 15, fov024.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.3.1.54 |
reduced ferredoxin |
reduced flavodoxin is te better cofactor |
Escherichia coli |
|
2.3.1.54 |
reduced flavodoxin |
a better electron donor than reduced ferredoxin |
Escherichia coli |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.3.1.54 |
gene pflB, the enzyme and its activating enzyme from Escherichia coli are expressed in a Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1. Enzyme PFL is expressed with two different electron donors, reduced ferredoxin or reduced flavodoxin, respectively. The coexpression of either of these electron donors has a positive effect on growth under aerobic conditions, indicating increased activity of PFL. |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.54 |
Escherichia coli |
P09373 |
gene pflB |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.3.1.54 |
PFL |
- |
Escherichia coli |
2.3.1.54 |
pyruvate formate lyase |
- |
Escherichia coli |
Expression
EC Number |
Organism |
Comment |
Expression |
---|
2.3.1.54 |
Escherichia coli |
coexpression of either of the electron donors reduced ferredoxin or reduced flavodoxin has a positive effect on growth under aerobic conditions, indicating increased activity of PFL. with a higher final biomass concentration and a significant increase in transcription of formate dehydrogenases |
up |