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Literature summary extracted from
- Desulfurization of alkylated forms of both dibenzothiophene and benzothiophene by a single bacterial strain (2000), FEMS Microbiol. Lett., 187, 123-126.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.21 | gene dszC, DNA and amino acid sequence determination and analysis, cloning and recombinant expression in Escherichia coli strain JM109 | Rhodococcus erythropolis |
| 1.14.14.22 | gene dszA, DNA and amino acid sequence determination and analysis | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.21 | 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | - |
2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? |  |
| 1.14.14.21 | 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis KA2-5-1 | - |
2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 2-ethyldibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | - |
2-ethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 2-ethyldibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis KA2-5-1 | - |
2-ethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3,4,6,7-tetramethyldibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | - |
3,4,6,7-tetramethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3,4,6,7-tetramethyldibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis KA2-5-1 | - |
3,4,6,7-tetramethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3,4,6-trimethyldibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | - |
3,4,6-trimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3-ethyldibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | - |
3-ethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | - |
4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4-methyldibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | - |
4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | benzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | - |
benzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | best substrate | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | additional information | Rhodococcus erythropolis | strain KA2-5-1 desulfurizes a variety of alkyl dibenzothiophenes through the specific cleavage of their C-S bonds. In addition, strain KA2-5-1 also attacks alkyl benzothiophenes in a C-S-bond-targeted fashion, substrate specificity overview | ? | - |
? | |
| 1.14.14.21 | additional information | Rhodococcus erythropolis KA2-5-1 | strain KA2-5-1 desulfurizes a variety of alkyl dibenzothiophenes through the specific cleavage of their C-S bonds. In addition, strain KA2-5-1 also attacks alkyl benzothiophenes in a C-S-bond-targeted fashion, substrate specificity overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.21 | Rhodococcus erythropolis | A0SWL2 | strain IGTS8, differs only in two positions from strain KA2-5-1 sequence; gene dszC | - |
| 1.14.14.21 | Rhodococcus erythropolis KA2-5-1 | A0SWL2 | strain IGTS8, differs only in two positions from strain KA2-5-1 sequence; gene dszC | - |
| 1.14.14.22 | Rhodococcus erythropolis | - |
gene dszA | - |
| 1.14.14.22 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
gene dszA | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.21 | cell culture | growth on dibenzothiophene as sole sulfur source, no or poor growth of strain KA2-5-1 with benzothiophene and various alkyl benzothiophenes, overview | Rhodococcus erythropolis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.21 | 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis KA2-5-1 | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 2-ethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | 2-ethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 2-ethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis KA2-5-1 | 2-ethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3,4,6,7-tetramethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | 3,4,6,7-tetramethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3,4,6,7-tetramethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis KA2-5-1 | 3,4,6,7-tetramethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3,4,6-trimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | 3,4,6-trimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3-ethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | 3-ethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4-methyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | benzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | benzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | best substrate | Rhodococcus erythropolis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | additional information | strain KA2-5-1 desulfurizes a variety of alkyl dibenzothiophenes through the specific cleavage of their C-S bonds. In addition, strain KA2-5-1 also attacks alkyl benzothiophenes in a C-S-bond-targeted fashion, substrate specificity overview | Rhodococcus erythropolis | ? | - |
? | |
| 1.14.14.21 | additional information | the purified monooxygenase, encoded by dszC of KA2-5-1, converts benzothiophene and dibenzothiophene into benzothiophene sulfone and dibenzothiophene sulfone, respectively, with the aid of an NADH-dependent oxidoreductase, substrate specificity overview | Rhodococcus erythropolis | ? | - |
? | |
| 1.14.14.21 | additional information | strain KA2-5-1 desulfurizes a variety of alkyl dibenzothiophenes through the specific cleavage of their C-S bonds. In addition, strain KA2-5-1 also attacks alkyl benzothiophenes in a C-S-bond-targeted fashion, substrate specificity overview | Rhodococcus erythropolis KA2-5-1 | ? | - |
? | |
| 1.14.14.21 | additional information | the purified monooxygenase, encoded by dszC of KA2-5-1, converts benzothiophene and dibenzothiophene into benzothiophene sulfone and dibenzothiophene sulfone, respectively, with the aid of an NADH-dependent oxidoreductase, substrate specificity overview | Rhodococcus erythropolis KA2-5-1 | ? | - |
? | |
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