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Literature summary extracted from

  • Ek, P.; Pettersson, G.; Ek, B.; Gong, F.; Li, J.P.; Zetterqvist, O.
    Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase (2002), Eur. J. Biochem., 269, 5016-5023.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.9.1.3 additional information insensitive towards okadaic acid and EDTA Homo sapiens
3.9.1.3 additional information insensitive towards okadaic acid and EDTA Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.9.1.3 cytosol
-
 Sus scrofa 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.9.1.3 13700
-
 x * 13700, mass spectrometry Sus scrofa
3.9.1.3 13700
-
 x * 13700, mass spectrometry Homo sapiens
3.9.1.3 13701
-
 x * 13701, calculated from amino acid sequence Sus scrofa
3.9.1.3 13701
-
 x * 13701, calculated from amino acid sequence Homo sapiens
3.9.1.3 14000
-
 x * 14000, SDS-PAGE Sus scrofa
3.9.1.3 14000
-
 x * 14000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.9.1.3 a [protein]-N-phospho-L-histidine + H2O Sus scrofa
-
 a [protein]-L-histidine + phosphate
-
 ?
3.9.1.3 a [protein]-N-phospho-L-histidine + H2O Homo sapiens
-
 a [protein]-L-histidine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.9.1.3 Homo sapiens Q9NRX4
-
-
3.9.1.3 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.9.1.3 DEAE-cellulose column chromatography, Sephadex G-200 gel filtration, Mono-Q column chromatography, and Superose 12 gel filtration Sus scrofa
3.9.1.3 DEAE-cellulose column chromatography, Sephadex G-200 gel filtration, Mono-Q column chromatography, and Superose 12 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.9.1.3 heart
-
 Homo sapiens
-
3.9.1.3 liver
-
 Sus scrofa
-
3.9.1.3 skeletal muscle
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.9.1.3 0.0028
-
 crude enzyme, at pH 7.5 and 30°C Sus scrofa
3.9.1.3 2.72
-
 after 971fold purification, at pH 7.5 and 30°C Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.9.1.3 a [protein]-N-phospho-L-histidine + H2O
-
 Sus scrofa a [protein]-L-histidine + phosphate
-
 ?
3.9.1.3 a [protein]-N-phospho-L-histidine + H2O
-
 Homo sapiens a [protein]-L-histidine + phosphate
-
 ?
3.9.1.3 succinyl-L-Ala-N-phospho-L-His-L-Pro-L-Phe 4-nitroanilide + H2O
-
 Sus scrofa succinyl-L-Ala-L-His-L-Pro-L-Phe 4-nitroanilide + phosphate
-
 ?
3.9.1.3 succinyl-L-Ala-N-phospho-L-His-L-Pro-L-Phe 4-nitroanilide + H2O
-
 Homo sapiens succinyl-L-Ala-L-His-L-Pro-L-Phe 4-nitroanilide + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.9.1.3 ? x * 14000, SDS-PAGE Sus scrofa
3.9.1.3 ? x * 14000, SDS-PAGE Homo sapiens
3.9.1.3 ? x * 13700, mass spectrometry Sus scrofa
3.9.1.3 ? x * 13700, mass spectrometry Homo sapiens
3.9.1.3 ? x * 13701, calculated from amino acid sequence Sus scrofa
3.9.1.3 ? x * 13701, calculated from amino acid sequence Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.9.1.3 7.5
-
-
 Sus scrofa
3.9.1.3 7.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.9.1.3 5.6 9 at pH 5.6, the activity is 2% of that at pH 7.5, and no activity is detected at pH 9.0 Sus scrofa
3.9.1.3 5.6 9 at pH 5.6, the activity is 2% of that at pH 7.5, and no activity is detected at pH 9.0 Homo sapiens