Literature summary extracted from
Hamed, R.B.; Gomez-Castellanos, J.R.; Froese, D.S.; Krysztofinska, E.; Yue, W.W.; Schofield, C.J.
Use of methylmalonyl-CoA epimerase in enhancing crotonase stereoselectivity (2015), ChemBioChem, 17, 1-7.
Application
EC Number |
Application |
Comment |
Organism |
---|
2.3.1.226 |
synthesis |
coupling of methylmalonyl-CoA epimerase, crotonyl-CoA carboxylase reductase and carboxymethylproline synthase mutants in a three-enzyme one-pot sequential synthesis of functionalised C-5 carboxyalkylprolines starting from crotonyl-CoA and carbon dioxide |
Pectobacterium carotovorum |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.3.1.226 |
M108V |
mutant is able to convert (2S)-methylmalonyl-CoA/L-glutamate semialdehyde/5-hydroxyproline/pyrroline-5-carboxylate to (6R)-6-methyl-(2S,5S)-5-carboxymethylproline |
Pectobacterium carotovorum |
2.3.1.226 |
W79F |
mutant is able to convert (2S)-ethylmalonyl-CoA/L-glutamate semialdehyde/5-hydroxyproline/pyrroline-5-carboxylate to (6R)-6-ethyl-(2S,5S)-5-carboxymethylproline |
Pectobacterium carotovorum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.226 |
Pectobacterium carotovorum |
Q9XB60 |
subsp. carotovorum |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.3.1.226 |
CarB |
- |
Pectobacterium carotovorum |