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Literature summary extracted from

  • Gaur, R.; Tiwari, S.
    Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07 (2015), BMC Biotechnol., 15, 19.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 2-mercaptoethanol 0.1%, 179% of initial activity Bacillus vallismortis
3.2.1.4 dithiothreitol 5 mM, 115% of initial activity Bacillus vallismortis
3.2.1.4 EDTA 5 mM, 121% of initial activity Bacillus vallismortis
3.2.1.4 iodoacetic acid 5 mM, 105% of initial activity Bacillus vallismortis
3.2.1.4 Urea 5 mM, 119% of initial activity Bacillus vallismortis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 4-chloromercuribenzoate 5 mM, 90% of initial activity Bacillus vallismortis
3.2.1.4 Hg2+ 5 mM, 46% residual activity Bacillus vallismortis
3.2.1.4 Mn2+ 5 mM, 77% residual activity Bacillus vallismortis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 carboxymethyl cellulose Km value 1.9 mg/ml, pH 7.0, 65°C Bacillus vallismortis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ 5 mM, 156% of initial activity Bacillus vallismortis
3.2.1.4 Fe2+ 5 mM, 113% of initial activity Bacillus vallismortis
3.2.1.4 Mg2+ 5 mM, 146% of initial activity Bacillus vallismortis
3.2.1.4 Na+ 5 mM, 128% of initial activity Bacillus vallismortis
3.2.1.4 Ni2+ 5 mM, 121% of initial activity Bacillus vallismortis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 80000
-
 x * 80000, SDS-PAGE Bacillus vallismortis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.4 benzene 30%, 1 h, no change in activity, 72 h, 109% of initial activity Bacillus vallismortis
3.2.1.4 Butanol 30%, 1 h, 121% of initial activity, 72 h, 122% of initial activity Bacillus vallismortis
3.2.1.4 isopropanol 30%, 1 h, 91% residual activity, 72 h, 92% residual activity Bacillus vallismortis
3.2.1.4 Methanol 30%, 1 h, no change in activity, 72 h, 125% of initial activity Bacillus vallismortis
3.2.1.4 n-decane 30%, 1 h, 135% of initial activity, 72 h, 158% of initial activity Bacillus vallismortis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Bacillus vallismortis
-
-
-
3.2.1.4 Bacillus vallismortis RG-07
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Bacillus vallismortis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 123600
-
 pH 7.0, 65°C Bacillus vallismortis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Bacillus vallismortis ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Bacillus vallismortis RG-07 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 80000, SDS-PAGE Bacillus vallismortis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 65
-
-
 Bacillus vallismortis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 95
-
 95% of maximum activity Bacillus vallismortis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 7
-
-
 Bacillus vallismortis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 9
-
 75% of maximum activtiy Bacillus vallismortis

Expression

EC Number Organism Comment Expression
3.2.1.4 Bacillus vallismortis maximum cellulase production upon growth on sugarcane baggase used as agro-waste carbon source additional information