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Literature summary extracted from

  • Sung, V.; Tsai, C.
    ADP-ribosylargininyl reaction of cholix toxin is mediated through diffusible intermediates (2014), BMC Biochem., 15, 26.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.31 expressed in Escherichia coli LC1062 cells Vibrio cholerae

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.31 E579Q the mutant shows reduced biotinylation intensity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 E581Q the mutant exhibits strongly reduced biotinylation intensity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R451K/R473K/R479K/R506K/R519K/R522K/R525K/R535K/R540K/R543K/R566K/R629K the mutant shows no auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R451K/R473K/R479K/R506K/R519K/R522K/R535K/R540K/R543K/R566K/R629K the mutant shows no auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R451K/R473K/R506K/R519K/R522K/R525K/R535K/R540K/R543K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and severely reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R451K/R473K/R506K/R519K/R522K/R535K/R540K/R543K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R451K/R473K/R506K/R519K/R522K/R540K/R543K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R473K/R479K/R506K/R519K/R522K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R473K/R506K/R519K/R522K/R540K/R543K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R473K/R506K/R519K/R522K/R540K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R473K/R506K/R519K/R522K/R543K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R473K/R506K/R519K/R522K/R566K/R629K the mutant shows increased auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R426K/R473K/R506K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R451A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R473A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R479A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R506A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R506K the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R506K/R566K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
2.4.2.31 R506Q the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R519A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R522A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R525A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R525K the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R525Q the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R530A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R530K inactive Vibrio cholerae
2.4.2.31 R530Q inactive Vibrio cholerae
2.4.2.31 R535A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R540A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R543A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R566A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 R629A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
2.4.2.31 Y493A inactive Vibrio cholerae
2.4.2.31 Y493A/E579Q/E581Q inactive Vibrio cholerae
2.4.2.31 Y493A/E581Q inactive Vibrio cholerae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.31 additional information Vibrio cholerae the enzyme also shows auto-ADP-ribosylation activity ?
-
 ?
2.4.2.31 NAD+ + protein L-arginine Vibrio cholerae
-
 nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.31 Vibrio cholerae Q5EK40
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.31 Ni-NTA column chromatography and Superdex 200 gel filtration Vibrio cholerae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.31 additional information the enzyme also shows auto-ADP-ribosylation activity Vibrio cholerae ?
-
 ?
2.4.2.31 NAD+ + eEF2 L-arginine
-
 Vibrio cholerae nicotinamide + Nomega-(ADP-D-ribosyl)-eEF2-L-arginine
-
 ?
2.4.2.31 NAD+ + protein L-arginine
-
 Vibrio cholerae nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
 ?
2.4.2.31 NAD+ + protein poly-L-arginine
-
 Vibrio cholerae nicotinamide + Nomega-(ADP-D-ribosyl)-protein-poly-L-arginine
-
 ?
2.4.2.31 NAD+ + protein R10H6 L-arginine
-
 Vibrio cholerae nicotinamide + Nomega-(ADP-D-ribosyl)-protein N10H6-L-arginine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.31 ADPRT
-
 Vibrio cholerae
2.4.2.31 cholix toxin
-
 Vibrio cholerae