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Literature summary extracted from

  • Jaito, N.; Saburi, W.; Odaka, R.; Kido, Y.; Hamura, K.; Nishimoto, M.; Kitaoka, M.; Matsui, H.; Mori, H.
    Characterization of a thermophilic 4-O-beta-D-mannosyl-D-glucose phosphorylase from Rhodothermus marinus (2014), Biosci. Biotechnol. Biochem., 78, 263-270.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.281 45000
-
 x * 45000, SDS-PAGE Rhodothermus marinus
2.4.1.281 222000
-
 PAGE Rhodothermus marinus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.281 Rhodothermus marinus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.281 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate = D-glucose + alpha-D-mannose 1-phosphate sequential bi-bi mechanism involving ternary complex formation Rhodothermus marinus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.281 1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate
-
 Rhodothermus marinus ? + phosphate
-
 r
2.4.1.281 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 Rhodothermus marinus D-glucose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.281 6-deoxy-D-glucose + alpha-D-mannose 1-phosphate
-
 Rhodothermus marinus 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
-
 r
2.4.1.281 D-glucose + alpha-D-mannose 1-phosphate
-
 Rhodothermus marinus 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 r
2.4.1.281 D-xylose + alpha-D-mannose 1-phosphate
-
 Rhodothermus marinus 4-O-beta-D-mannopyranosyl-D-xylopyranose + phosphate
-
 r
2.4.1.281 methyl beta-D-glucoside + alpha-D-mannose 1-phosphate
-
 Rhodothermus marinus methyl [4-O-beta-D-mannopyranosyl]-beta-D-glucopyranoside + phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.281 multimer x * 45000, SDS-PAGE Rhodothermus marinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.281 75
-
-
 Rhodothermus marinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.281 80
-
 stable below Rhodothermus marinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.281 6.5
-
-
 Rhodothermus marinus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.281 5.5 8.3
-
 Rhodothermus marinus

Expression

EC Number Organism Comment Expression
2.4.1.281 Rhodothermus marinus expression is increased in presence of Konjac glucomannan up