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Literature summary extracted from

  • Yamada, S.; Arikawa, S.
    An ectotherm homologue of human predicted gene NAT16 encodes histidine N-acetyltransferase responsible for Nalpha-acetylhistidine synthesis (2014), Biochim. Biophys. Acta, 1840, 434-442.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.33 gene hisat, DNA and amino acid sequence determination and analysis Oreochromis niloticus
2.3.1.33 gene hisat, DNA and amino acid sequence determination and analysis, the HISAT gene is expressed as two splice variants containing an identical ORF but differing lengths of 5'-UTR. Both variants are expressed exclusively in the fish brain and lens, recombinant expression in a cell-free translation system, and recombinant expression of the two HISAT splice variant-1 and -2 in Oreochromis niloticus Scomber australasicus
2.3.1.33 gene Nat16, DNA and amino acid sequence determination and analysis, recombinant expression in a cell-free translation system Homo sapiens

General Stability

EC Number General Stability Organism
2.3.1.33 HISAT is an unstable enzyme that is rapidly and irreversibly inactivated during preincubation at 37 °C in the absence of acetyl-CoA Scomber australasicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.33 39000
-
 gel filtration Scomber australasicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.33 acetyl-CoA + L-histidine Scomber australasicus
-
 CoA + N-acetyl-L-histidine
-
 ?
2.3.1.33 acetyl-CoA + L-histidine Homo sapiens
-
 CoA + N-acetyl-L-histidine
-
 ?
2.3.1.33 acetyl-CoA + L-histidine Oreochromis niloticus
-
 CoA + N-acetyl-L-histidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.33 Homo sapiens Q8N8M0 gene Nat16
-
2.3.1.33 Oreochromis niloticus I3J7Q8 gene hisat, two HISAT splice variant-1 and -2
-
2.3.1.33 Scomber australasicus U3U715 gene hisat, two HISAT splice variant-1 and -2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.33 native enzyme 4453fold from brain by ammonium sulfate fractionation, dye-ligand affinity chromatography, gel filtration, and anion exchange chromatography followed by ultrafiltration Scomber australasicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.33 brain
-
 Scomber australasicus
-
2.3.1.33 brain
-
 Homo sapiens
-
2.3.1.33 lens
-
 Scomber australasicus
-
2.3.1.33 additional information the HISAT gene is expressed as two splice variants containing an identical ORF but differing lengths of 5'-UTR. Both variants are expressed exclusively in the fish brain and lens Scomber australasicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.33 1692
-
 purified native enzyme, pH 7.8, 37°C Scomber australasicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.33 acetyl-CoA + L-histidine
-
 Scomber australasicus CoA + N-acetyl-L-histidine
-
 ?
2.3.1.33 acetyl-CoA + L-histidine
-
 Homo sapiens CoA + N-acetyl-L-histidine
-
 ?
2.3.1.33 acetyl-CoA + L-histidine
-
 Oreochromis niloticus CoA + N-acetyl-L-histidine
-
 ?
2.3.1.33 additional information the recombinant human NAT16 protein has only trace enzyme activity for N-acetyl-L-histidine synthesis Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.33 HISAT
-
 Scomber australasicus
2.3.1.33 HISAT
-
 Homo sapiens
2.3.1.33 HISAT
-
 Oreochromis niloticus
2.3.1.33 N-acetyltransferase 16
-
 Homo sapiens
2.3.1.33 N-acetyltransferase 16
-
 Oreochromis niloticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.33 37
-
 assay at Scomber australasicus
2.3.1.33 37
-
 assay at Homo sapiens
2.3.1.33 37
-
 assay at Oreochromis niloticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.33 37
-
 the purified enzyme HISAT is an unstable enzyme that is rapidly and irreversibly inactivated during preincubation at 37 °C in the absence of acetyl-CoA Scomber australasicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.33 7.8
-
 assay at Scomber australasicus
2.3.1.33 7.8
-
 assay at Homo sapiens
2.3.1.33 7.8
-
 assay at Oreochromis niloticus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.33 acetyl-CoA
-
 Scomber australasicus
2.3.1.33 acetyl-CoA
-
 Homo sapiens
2.3.1.33 acetyl-CoA
-
 Oreochromis niloticus

General Information

EC Number General Information Comment Organism
2.3.1.33 additional information partial amino acid sequences determination and analysis using mass spectrometry and Edman degradation Scomber australasicus
2.3.1.33 additional information partial amino acid sequences determination and analysis using mass spectrometry and Edman degradation Homo sapiens