BRENDA - Enzyme Database show

Mechanistic and structural analysis of Drosophila melanogaster arylalkylamine N-acetyltransferases

Dempsey, D.R.; Jeffries, K.A.; Bond, J.D.; Carpenter, A.M.; Rodriguez-Ospina, S.; Breydo, L.; Caswell, K.K.; Merkler, D.J.; Biochemistry 53, 7777-7793 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.87
expression in Escherichia coli
Drosophila melanogaster
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.87
E26A
22-32fold decrease in the kcat relative to that of the wild type, catalytic efficiency is less than 10% of wild-type. Residue E26 is the general base in catalysis
Drosophila melanogaster
2.3.1.87
H206A
3-4-old decrease in the kcat and an increase in the Km for both acetyl-CoA (7fold) and histamine (4fold)
Drosophila melanogaster
2.3.1.87
P27A
38-42fold decrease in the kcat
Drosophila melanogaster
2.3.1.87
R138A
2.4-2.8fold increase in kcat
Drosophila melanogaster
2.3.1.87
S171A
catalytic efficiency for acetyl-CoA and histamine are only 42 and 5.2% of that of the wild-type enzyme, respectively
Drosophila melanogaster
2.3.1.87
T167A
16-17fold decrease in kcat, catalytic efficiency is about 1% of wild-type
Drosophila melanogaster
2.3.1.87
T167A/S171A
catalytic efficiency for acetyl-CoA and histamine are only 5.1 and 0.28% of that of the wild-type enzyme, respectively
Drosophila melanogaster
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.87
0.0023
-
histamine
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.0034
-
histamine
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.0049
-
histamine
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.006
-
butanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.0067
-
histamine
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.009
-
5-benzyloxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.013
-
acetyl-CoA
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.013
-
histamine
mutant T167A/S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.016
-
histamine
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.019
-
acetyl-CoA
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.026
-
tryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.029
-
acetyl-CoA
mutant T167A/S171A, pH 8.0, 22°C; wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.04
-
hexanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.042
-
5-methoxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.042
-
tyramine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.061
-
3-methoxyphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.12
-
octopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.14
-
acetyl-CoA
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.16
-
serotonin
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.17
-
dopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.18
-
3-(trifluoromethyl)phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.21
-
acetyl-CoA
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.26
-
beta-methylphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.32
-
phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.52
-
histamine
cosubstrate acetyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.55
-
acetyl-CoA
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
2.9
-
histamine
cosubstrate butanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
15
-
histamine
cosubstrate hexanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.87
Drosophila melanogaster
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.87
acetyl-CoA + 3-(trifluoromethyl)phenethylamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-3-(trifluoromethyl)phenethylamine
-
-
-
?
2.3.1.87
acetyl-CoA + 3-methoxyphenethylamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-3-methoxyphenethylamine
-
-
-
?
2.3.1.87
acetyl-CoA + 5-benzyloxytryptamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-5-benzyloxytryptamine
-
-
-
?
2.3.1.87
acetyl-CoA + 5-methoxytryptamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-5-methoxytryptamine
-
-
-
?
2.3.1.87
acetyl-CoA + beta-methylphenethylamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-beta-methylphenethylamine
-
-
-
?
2.3.1.87
acetyl-CoA + dopamine
-
735681
Drosophila melanogaster
CoA + N-acetyldopamine
-
-
-
?
2.3.1.87
acetyl-CoA + histamine
-
735681
Drosophila melanogaster
CoA + N-acetylhistamine
-
-
-
?
2.3.1.87
acetyl-CoA + octopamine
-
735681
Drosophila melanogaster
CoA + N-acetyloctopamine
-
-
-
?
2.3.1.87
acetyl-CoA + phenethylamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-phenethylamine
-
-
-
?
2.3.1.87
acetyl-CoA + serotonin
-
735681
Drosophila melanogaster
CoA + N-acetylserotonin
-
-
-
?
2.3.1.87
acetyl-CoA + tryptamine
-
735681
Drosophila melanogaster
CoA + N-acetyltryptamine
-
-
-
?
2.3.1.87
acetyl-CoA + tyramine
-
735681
Drosophila melanogaster
CoA + N-acetyltyramine
-
-
-
?
2.3.1.87
butanoyl-CoA + histamine
-
735681
Drosophila melanogaster
CoA + N-butanoylhistamine
-
-
-
?
2.3.1.87
hexanoyl-CoA + histamine
-
735681
Drosophila melanogaster
CoA + N-hexanoylhistamine
-
-
-
?
2.3.1.87
additional information
ordered sequential mechanism, with acetyl-CoA binding first followed by histamine to generate an AANATL7-acetyl-CoA-histamine ternary complex prior to catalysis. An ionizable group with a pKa of 7.1 is assigned to Glu-26 as a general base and a second with a pKa of 9.5 is assigned to the protonation of the thiolate of the coenzyme A product
735681
Drosophila melanogaster
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.87
0.093
-
hexanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.1
-
histamine
cosubstrate hexanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.66
-
butanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.87
-
histamine
cosubstrate butanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1.01
-
acetyl-CoA
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1.39
-
histamine
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1.61
-
acetyl-CoA
mutant T167A/S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1.84
-
acetyl-CoA
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1.9
-
histamine
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
2.2
-
histamine
mutant T167A/S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
5.7
-
5-benzyloxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
7.7
-
histamine
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
8.6
-
acetyl-CoA
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
9.6
-
acetyl-CoA
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
10.4
-
histamine
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
13.1
-
5-methoxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
16
-
octopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
16
-
tryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
17
-
serotonin
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
21
-
dopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
26
-
3-methoxyphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
29
-
beta-methylphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
30
-
histamine
cosubstrate acetyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
32
-
acetyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
40
-
3-(trifluoromethyl)phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
42
-
phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
71
-
histamine
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
88
-
acetyl-CoA
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
238
-
tyramine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.87
expression in Escherichia coli
Drosophila melanogaster
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.87
E26A
22-32fold decrease in the kcat relative to that of the wild type, catalytic efficiency is less than 10% of wild-type. Residue E26 is the general base in catalysis
Drosophila melanogaster
2.3.1.87
H206A
3-4-old decrease in the kcat and an increase in the Km for both acetyl-CoA (7fold) and histamine (4fold)
Drosophila melanogaster
2.3.1.87
P27A
38-42fold decrease in the kcat
Drosophila melanogaster
2.3.1.87
R138A
2.4-2.8fold increase in kcat
Drosophila melanogaster
2.3.1.87
S171A
catalytic efficiency for acetyl-CoA and histamine are only 42 and 5.2% of that of the wild-type enzyme, respectively
Drosophila melanogaster
2.3.1.87
T167A
16-17fold decrease in kcat, catalytic efficiency is about 1% of wild-type
Drosophila melanogaster
2.3.1.87
T167A/S171A
catalytic efficiency for acetyl-CoA and histamine are only 5.1 and 0.28% of that of the wild-type enzyme, respectively
Drosophila melanogaster
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.87
0.0023
-
histamine
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.0034
-
histamine
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.0049
-
histamine
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.006
-
butanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.0067
-
histamine
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.009
-
5-benzyloxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.013
-
acetyl-CoA
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.013
-
histamine
mutant T167A/S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.016
-
histamine
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.019
-
acetyl-CoA
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.026
-
tryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.029
-
acetyl-CoA
mutant T167A/S171A, pH 8.0, 22°C; wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.04
-
hexanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.042
-
5-methoxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.042
-
tyramine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.061
-
3-methoxyphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.12
-
octopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.14
-
acetyl-CoA
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.16
-
serotonin
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.17
-
dopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.18
-
3-(trifluoromethyl)phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.21
-
acetyl-CoA
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.26
-
beta-methylphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.32
-
phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.52
-
histamine
cosubstrate acetyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.55
-
acetyl-CoA
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
2.9
-
histamine
cosubstrate butanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
15
-
histamine
cosubstrate hexanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.87
acetyl-CoA + 3-(trifluoromethyl)phenethylamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-3-(trifluoromethyl)phenethylamine
-
-
-
?
2.3.1.87
acetyl-CoA + 3-methoxyphenethylamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-3-methoxyphenethylamine
-
-
-
?
2.3.1.87
acetyl-CoA + 5-benzyloxytryptamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-5-benzyloxytryptamine
-
-
-
?
2.3.1.87
acetyl-CoA + 5-methoxytryptamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-5-methoxytryptamine
-
-
-
?
2.3.1.87
acetyl-CoA + beta-methylphenethylamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-beta-methylphenethylamine
-
-
-
?
2.3.1.87
acetyl-CoA + dopamine
-
735681
Drosophila melanogaster
CoA + N-acetyldopamine
-
-
-
?
2.3.1.87
acetyl-CoA + histamine
-
735681
Drosophila melanogaster
CoA + N-acetylhistamine
-
-
-
?
2.3.1.87
acetyl-CoA + octopamine
-
735681
Drosophila melanogaster
CoA + N-acetyloctopamine
-
-
-
?
2.3.1.87
acetyl-CoA + phenethylamine
-
735681
Drosophila melanogaster
CoA + N-acetyl-phenethylamine
-
-
-
?
2.3.1.87
acetyl-CoA + serotonin
-
735681
Drosophila melanogaster
CoA + N-acetylserotonin
-
-
-
?
2.3.1.87
acetyl-CoA + tryptamine
-
735681
Drosophila melanogaster
CoA + N-acetyltryptamine
-
-
-
?
2.3.1.87
acetyl-CoA + tyramine
-
735681
Drosophila melanogaster
CoA + N-acetyltyramine
-
-
-
?
2.3.1.87
butanoyl-CoA + histamine
-
735681
Drosophila melanogaster
CoA + N-butanoylhistamine
-
-
-
?
2.3.1.87
hexanoyl-CoA + histamine
-
735681
Drosophila melanogaster
CoA + N-hexanoylhistamine
-
-
-
?
2.3.1.87
additional information
ordered sequential mechanism, with acetyl-CoA binding first followed by histamine to generate an AANATL7-acetyl-CoA-histamine ternary complex prior to catalysis. An ionizable group with a pKa of 7.1 is assigned to Glu-26 as a general base and a second with a pKa of 9.5 is assigned to the protonation of the thiolate of the coenzyme A product
735681
Drosophila melanogaster
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.87
0.093
-
hexanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.1
-
histamine
cosubstrate hexanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.66
-
butanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.87
-
histamine
cosubstrate butanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1.01
-
acetyl-CoA
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1.39
-
histamine
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1.61
-
acetyl-CoA
mutant T167A/S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1.84
-
acetyl-CoA
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1.9
-
histamine
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
2.2
-
histamine
mutant T167A/S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
5.7
-
5-benzyloxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
7.7
-
histamine
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
8.6
-
acetyl-CoA
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
9.6
-
acetyl-CoA
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
10.4
-
histamine
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
13.1
-
5-methoxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
16
-
octopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
16
-
tryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
17
-
serotonin
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
21
-
dopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
26
-
3-methoxyphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
29
-
beta-methylphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
30
-
histamine
cosubstrate acetyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
32
-
acetyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
40
-
3-(trifluoromethyl)phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
42
-
phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
71
-
histamine
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
88
-
acetyl-CoA
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
238
-
tyramine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.3.1.87
0.007
-
histamine
cosubstrate hexanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.086
-
histamine
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.16
-
histamine
mutant T167A/S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.23
-
hexanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.28
-
histamine
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.38
-
histamine
cosubstrate butanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
3
-
histamine
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
3.3
-
histamine
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
13.6
-
acetyl-CoA
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
15
-
histamine
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
46
-
acetyl-CoA
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
56
-
acetyl-CoA
mutant T167A/S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
58
-
histamine
cosubstrate acetyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
77
-
acetyl-CoA
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
110
-
beta-methylphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
110
-
butanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
110
-
serotonin
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
120
-
dopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
130
-
octopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
130
-
phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
160
-
acetyl-CoA
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
220
-
3-(trifluoromethyl)phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
310
-
5-methoxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
430
-
3-methoxyphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
460
-
acetyl-CoA
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
550
-
tyramine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
600
-
tryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
630
-
5-benzyloxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1100
-
acetyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.3.1.87
0.007
-
histamine
cosubstrate hexanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.086
-
histamine
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.16
-
histamine
mutant T167A/S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.23
-
hexanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.28
-
histamine
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
0.38
-
histamine
cosubstrate butanoyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
3
-
histamine
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
3.3
-
histamine
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
13.6
-
acetyl-CoA
mutant T167A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
15
-
histamine
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
46
-
acetyl-CoA
mutant H206A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
56
-
acetyl-CoA
mutant T167A/S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
58
-
histamine
cosubstrate acetyl-CoA, wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
77
-
acetyl-CoA
mutant E26A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
110
-
beta-methylphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
110
-
butanoyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
110
-
serotonin
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
120
-
dopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
130
-
octopamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
130
-
phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
160
-
acetyl-CoA
mutant R138A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
220
-
3-(trifluoromethyl)phenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
310
-
5-methoxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
430
-
3-methoxyphenethylamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
460
-
acetyl-CoA
mutant S171A, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
550
-
tyramine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
600
-
tryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
630
-
5-benzyloxytryptamine
wild-type, pH 8.0, 22°C
Drosophila melanogaster
2.3.1.87
1100
-
acetyl-CoA
wild-type, pH 8.0, 22°C
Drosophila melanogaster