EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.13 | expressed in Escherichia coli BL21(DE3)pLysS cells | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | L-leucine | partially inhibited by L-leucine | Methanocaldococcus jannaschii | |
2.3.3.13 | Mg2+ | inhibitory at 20 mM | Methanocaldococcus jannaschii | |
2.3.3.13 | Mn2+ | inhibitory at 20 mM | Methanocaldococcus jannaschii | |
2.3.3.13 | Zn2+ | inhibitory at 20 mM | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.07 | - |
acetyl-CoA | at pH 7.8 and 37°C | Methanocaldococcus jannaschii | |
2.3.3.13 | 0.09 | - |
3-methyl-2-oxobutanoate | at pH 7.8 and 37°C | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | K+ | enzyme activity increases 1.5fold in the presence of 100 mM KCl or NaCl | Methanocaldococcus jannaschii | |
2.3.3.13 | Na+ | enzyme activity increases 1.5fold in the presence of 100 mM KCl or NaCl | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Methanocaldococcus jannaschii | - |
(2S)-2-isopropylmalate + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.13 | Methanocaldococcus jannaschii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.13 | Ni2+ HisTrap column chromatography | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Methanocaldococcus jannaschii | (2S)-2-isopropylmalate + CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.13 | Alpha-isopropylmalate synthase | - |
Methanocaldococcus jannaschii |
2.3.3.13 | IPMS | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 90 | - |
the enzyme retains full activity after incubation in water for 15 min at up to 90°C | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 330 | - |
3-methyl-2-oxobutanoate | at pH 7.8 and 37°C | Methanocaldococcus jannaschii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.16 | - |
L-leucine | at pH 7.8 and 37 °C | Methanocaldococcus jannaschii |