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Literature summary extracted from

  • Casey, A.K.; Hicks, M.A.; Johnson, J.L.; Babbitt, P.C.; Frantom, P.A.
    Mechanistic and bioinformatic investigation of a conserved active site helix in alpha-isopropylmalate synthase from Mycobacterium tuberculosis, a member of the DRE-TIM metallolyase superfamily (2014), Biochemistry, 53, 2915-2925.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.13 expressed in Escherichia coli BL21(DE3)pLysS cells Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.13 L-leucine partially inhibited by L-leucine Methanocaldococcus jannaschii
2.3.3.13 Mg2+ inhibitory at 20 mM Methanocaldococcus jannaschii
2.3.3.13 Mn2+ inhibitory at 20 mM Methanocaldococcus jannaschii
2.3.3.13 Zn2+ inhibitory at 20 mM Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.13 0.07
-
acetyl-CoA at pH 7.8 and 37°C Methanocaldococcus jannaschii
2.3.3.13 0.09
-
3-methyl-2-oxobutanoate at pH 7.8 and 37°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.13 K+ enzyme activity increases 1.5fold in the presence of 100 mM KCl or NaCl Methanocaldococcus jannaschii
2.3.3.13 Na+ enzyme activity increases 1.5fold in the presence of 100 mM KCl or NaCl Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Methanocaldococcus jannaschii
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.13 Ni2+ HisTrap column chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Methanocaldococcus jannaschii (2S)-2-isopropylmalate + CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.13 Alpha-isopropylmalate synthase
-
Methanocaldococcus jannaschii
2.3.3.13 IPMS
-
Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.13 90
-
the enzyme retains full activity after incubation in water for 15 min at up to 90°C Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.13 330
-
3-methyl-2-oxobutanoate at pH 7.8 and 37°C Methanocaldococcus jannaschii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.13 0.16
-
L-leucine at pH 7.8 and 37 °C Methanocaldococcus jannaschii